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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-37488 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal eIF4B Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: eIF4B Antibody (1F5) [NBP2-37488] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: eIF4B Antibody (1F5) [NBP2-37488] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: eIF4B Antibody (1F5) [NBP2-37488] - Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).
