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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB3774 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3774, RRID:AB_1574631
- Product name
- EIF4B polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EIF4B.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Herpes simplex virus virion host shutoff protein is stimulated by translation initiation factors eIF4B and eIF4H.
Solution structure and RNA interactions of the RNA recognition motif from eukaryotic translation initiation factor 4B.
Cloning and expression of eukaryotic initiation factor 4B cDNA: sequence determination identifies a common RNA recognition motif.
Doepker RC, Hsu WL, Saffran HA, Smiley JR
Journal of virology 2004 May;78(9):4684-99
Journal of virology 2004 May;78(9):4684-99
Solution structure and RNA interactions of the RNA recognition motif from eukaryotic translation initiation factor 4B.
Fleming K, Ghuman J, Yuan X, Simpson P, Szendröi A, Matthews S, Curry S
Biochemistry 2003 Aug 5;42(30):8966-75
Biochemistry 2003 Aug 5;42(30):8966-75
Cloning and expression of eukaryotic initiation factor 4B cDNA: sequence determination identifies a common RNA recognition motif.
Milburn SC, Hershey JW, Davies MV, Kelleher K, Kaufman RJ
The EMBO journal 1990 Sep;9(9):2783-90
The EMBO journal 1990 Sep;9(9):2783-90
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4B polyclonal antibody (Cat # PAB3774) in HepG2 cell line lysates (35 ug/lane). EIF4B (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4B polyclonal antibody (Cat # PAB3774) in 293 cell line lysates transiently transfected with the EIF4B gene (2 ug/lane). EIF4B (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B polyclonal antibody (Cat # PAB3774) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
