PA5-29540

antibody from Invitrogen Antibodies

Targeting: EIF4B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29540

Provider

Invitrogen Antibodies

Product name

eIF4B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: NT2D1, IMR32, MCF-7, U87-MG, SK-N-SH, SK-N-AS, 293T, A431, HeLa, HepG2, human EIF4B-transfected 293T.

Concentration

0.16 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EIF4B antibody detects EIF4B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5 % SDS-PAGE, and blotted with EIF4B antibody eIF4B Polyclonal Antibody (Product # PA5-29540) diluted by 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EIF4B antibody detects EIF4B protein by western blot analysis. A. 30 µg 293T whole cell extract. B. 30 µg whole cell extract of human EIF4B-transfected 293T cells.7.5 % SDS-PAGE. EIF4B antibody eIF4B Polyclonal Antibody (Product # PA5-29540) dilution: 1:5,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using eIF4B Polyclonal Antibody (Product # PA5-29540). Sample (30 µg of whole cell lysate). Lane A: NT2D1. Lane B: IMR32. Lane C: U87-MG. Lane D: MCF-7 . 7.5% SDS PAGE. eIF4B Polyclonal Antibody (Product # PA5-29540) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EIF4B antibody detects EIF4B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EIF4B antibody eIF4B Polyclonal Antibody (Product # PA5-29540) diluted by 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EIF4B antibody detects EIF4B protein by western blot analysis. A. 30 µg 293T whole cell extract. B. 30 µg whole cell extract of human EIF4B-transfected 293T cells.7.5 % SDS-PAGE. EIF4B antibody eIF4B Polyclonal Antibody (Product # PA5-29540) dilution: 1:5,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of EIF4B in paraformaldehyde-fixed MCF-7 cells using an EIF4B polyclonal antibody (Product # PA5-29540) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EIF4B antibody [N1N2], N-term detects EIF4B protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. EIF4B antibody [N1N2], N-term (Product # PA5-29540) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.