Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA1-067 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha COP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-067 detects coatomer-protein I alpha (COPI alpha) from human and rat cells. PA1-067 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~140 kDa protein representing COP alpha from PC-12 cell extract. This antibody also detects an unidentified protein at ~100 kDa. Immunocytochemical staining of alpha-COP using PA1-067 results in alpha-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm. PA1-067 immunogen is a synthetic peptide corresponding to residues M(1) L T K F E T K S A R V K G L S F H P(19) C of rat alpha-COP. The PA1-067 immunizing peptide (Cat. # PEP-125) is available for use in neutralization and control experiments.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Intracellular trafficking of KA2 kainate receptors mediated by interactions with coatomer protein complex I (COPI) and 14-3-3 chaperone systems.
The human phosphatidylinositol phosphatase SAC1 interacts with the coatomer I complex.
Vivithanaporn P, Yan S, Swanson GT
The Journal of biological chemistry 2006 Jun 2;281(22):15475-84
The Journal of biological chemistry 2006 Jun 2;281(22):15475-84
The human phosphatidylinositol phosphatase SAC1 interacts with the coatomer I complex.
Rohde HM, Cheong FY, Konrad G, Paiha K, Mayinger P, Boehmelt G
The Journal of biological chemistry 2003 Dec 26;278(52):52689-99
The Journal of biological chemistry 2003 Dec 26;278(52):52689-99
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunolocalization of COP alpha in HeLa cells using Product # PA1-067.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL