Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- GTX19389 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX19389, RRID:AB_423561
- Product name
- Her3 / ErbB3 antibody [RTJ2]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Flow cytometry analysis of ErbB3 in HeLa cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ErbB3 antibody [RTJ2] at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a proper secondary antibody and re-suspended in PBS for FACS analysis.