Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-13040 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ErbB3 Monoclonal Antibody (2B5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-13040 targets HER-3 in IP and WB applications and shows reactivity with Human, mouse, and Rat samples.
- Antibody clone number
- 2B5
- Concentration
- 0.2 mg/mL
Submitted references Interaction with epsin 1 regulates the constitutive clathrin-dependent internalization of ErbB3.
Soluble-E-cadherin activates HER and IAP family members in HER2+ and TNBC human breast cancers.
Oligomerization of the Nrdp1 E3 ubiquitin ligase is necessary for efficient autoubiquitination but not ErbB3 ubiquitination.
HER3, p95HER2, and HER2 protein expression levels define multiple subtypes of HER2-positive metastatic breast cancer.
Phosphoinositide 3-kinase pathway activation in phosphate and tensin homolog (PTEN)-deficient prostate cancer cells is independent of receptor tyrosine kinases and mediated by the p110beta and p110delta catalytic subunits.
The constitutive activity of epidermal growth factor receptor vIII leads to activation and differential trafficking of wild-type epidermal growth factor receptor and erbB2.
The ectodomain shedding of E-cadherin by ADAM15 supports ErbB receptor activation.
Activation of nonreceptor tyrosine kinase Bmx/Etk mediated by phosphoinositide 3-kinase, epidermal growth factor receptor, and ErbB3 in prostate cancer cells.
The ErbB4 receptor in fetal rat lung fibroblasts and epithelial type II cells.
ErbB4 regulates fetal surfactant phospholipid synthesis in primary fetal rat type II cells.
ErbB receptor dimerization, localization, and co-localization in mouse lung type II epithelial cells.
Adult rat otic placode-derived neurons and sensory epithelium express all four erbB receptors: a role in regulating vestibular ganglion neuron viability.
Szymanska M, Fosdahl AM, Raiborg C, Dietrich M, Liestøl K, Stang E, Bertelsen V
Biochimica et biophysica acta 2016 Jun;1863(6 Pt A):1179-88
Biochimica et biophysica acta 2016 Jun;1863(6 Pt A):1179-88
Soluble-E-cadherin activates HER and IAP family members in HER2+ and TNBC human breast cancers.
Brouxhon SM, Kyrkanides S, Teng X, O'Banion MK, Clarke R, Byers S, Ma L
Molecular carcinogenesis 2014 Nov;53(11):893-906
Molecular carcinogenesis 2014 Nov;53(11):893-906
Oligomerization of the Nrdp1 E3 ubiquitin ligase is necessary for efficient autoubiquitination but not ErbB3 ubiquitination.
Printsev I, Yen L, Sweeney C, Carraway KL 3rd
The Journal of biological chemistry 2014 Mar 21;289(12):8570-8
The Journal of biological chemistry 2014 Mar 21;289(12):8570-8
HER3, p95HER2, and HER2 protein expression levels define multiple subtypes of HER2-positive metastatic breast cancer.
Lipton A, Goodman L, Leitzel K, Cook J, Sperinde J, Haddad M, Köstler WJ, Huang W, Weidler JM, Ali S, Newton A, Fuchs EM, Paquet A, Singer CF, Horvat R, Jin X, Banerjee J, Mukherjee A, Tan Y, Shi Y, Chenna A, Larson J, Lie Y, Sherwood T, Petropoulos CJ, Williams S, Winslow J, Parry G, Bates M
Breast cancer research and treatment 2013 Aug;141(1):43-53
Breast cancer research and treatment 2013 Aug;141(1):43-53
Phosphoinositide 3-kinase pathway activation in phosphate and tensin homolog (PTEN)-deficient prostate cancer cells is independent of receptor tyrosine kinases and mediated by the p110beta and p110delta catalytic subunits.
Jiang X, Chen S, Asara JM, Balk SP
The Journal of biological chemistry 2010 May 14;285(20):14980-14989
The Journal of biological chemistry 2010 May 14;285(20):14980-14989
The constitutive activity of epidermal growth factor receptor vIII leads to activation and differential trafficking of wild-type epidermal growth factor receptor and erbB2.
Zeineldin R, Ning Y, Hudson LG
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2010 Jun;58(6):529-41
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2010 Jun;58(6):529-41
The ectodomain shedding of E-cadherin by ADAM15 supports ErbB receptor activation.
Najy AJ, Day KC, Day ML
The Journal of biological chemistry 2008 Jun 27;283(26):18393-401
The Journal of biological chemistry 2008 Jun 27;283(26):18393-401
Activation of nonreceptor tyrosine kinase Bmx/Etk mediated by phosphoinositide 3-kinase, epidermal growth factor receptor, and ErbB3 in prostate cancer cells.
Jiang X, Borgesi RA, McKnight NC, Kaur R, Carpenter CL, Balk SP
The Journal of biological chemistry 2007 Nov 9;282(45):32689-98
The Journal of biological chemistry 2007 Nov 9;282(45):32689-98
The ErbB4 receptor in fetal rat lung fibroblasts and epithelial type II cells.
Liu W, Zscheppang K, Murray S, Nielsen HC, Dammann CE
Biochimica et biophysica acta 2007 Jul;1772(7):737-47
Biochimica et biophysica acta 2007 Jul;1772(7):737-47
ErbB4 regulates fetal surfactant phospholipid synthesis in primary fetal rat type II cells.
Zscheppang K, Liu W, Volpe MV, Nielsen HC, Dammann CE
American journal of physiology. Lung cellular and molecular physiology 2007 Aug;293(2):L429-35
American journal of physiology. Lung cellular and molecular physiology 2007 Aug;293(2):L429-35
ErbB receptor dimerization, localization, and co-localization in mouse lung type II epithelial cells.
Zscheppang K, Korenbaum E, Bueter W, Ramadurai SM, Nielsen HC, Dammann CE
Pediatric pulmonology 2006 Dec;41(12):1205-12
Pediatric pulmonology 2006 Dec;41(12):1205-12
Adult rat otic placode-derived neurons and sensory epithelium express all four erbB receptors: a role in regulating vestibular ganglion neuron viability.
Matsunaga T, Davis JG, Greene MI
DNA and cell biology 2001 Jun;20(6):307-19
DNA and cell biology 2001 Jun;20(6):307-19
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of HER-3 using HER-3 Monoclonal Antibody (Product # MA5-13040) on T47D Cells and MAD109 Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of ErbB3 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR764358_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of ErbB3 was performed by loading 60 µg of T-47D wild type (Lane 1), T-47D Cas9 (Lane 2) andT-47D ERBB3 KO (Lane 3) membrane enriched extracts. The samples were electrophoresed using NuPAGE™ 3 to 8%, Tris-Acetate, 1.0 mm, Mini Protein Gel (Product # EC6695BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with ErbB3 Monoclonal Antibody (2B5) (Product # MA5-13040, 1:100 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:10.000 dilution) using the iBright™ FL 1500 (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to ErbB3. Uncharacterized bands was observed in wild type and Cas9 lanes at ~71 kDa and ~150 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HER-3 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HER-3 (2B5) Mouse Monoclonal Antibody (Product # MA5-13040) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300).Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HER-3 using HER-3 Monoclonal Antibody (Product # MA5-13040) on denatured Human T47D Cells.