PAB11256

antibody from Abnova Corporation

Targeting: RNF25 AO7, FLJ13906

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB11256

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB11256, RRID:AB_1713086

Product name

RNF25 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of RNF25.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

RING finger protein AO7 supports NF-kappaB-mediated transcription by interacting with the transactivation domain of the p65 subunit.

Asamitsu K, Tetsuka T, Kanazawa S, Okamoto T
The Journal of biological chemistry 2003 Jul 18;278(29):26879-87

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RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination.

Lorick KL, Jensen JP, Fang S, Ong AM, Hatakeyama S, Weissman AM
Proceedings of the National Academy of Sciences of the United States of America 1999 Sep 28;96(20):11364-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using RNF25 polyclonal antibody (Cat # PAB11256) shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 3 is a vector only control. The primary antibody diluted to 1 : 1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

1 ug of RNF25 polyclonal antibody (Cat # PAB11256) was used in immunoprecipitation of 20 ug HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.

Validation comment

Immunoprecipitation