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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00064320-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00064320-D01, RRID:AB_10731569
- Product name
- RNF25 MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human RNF25 protein.
- Antigen sequence
MAASASAAAGEEDWVLPSEVEVLESIYLDELQVIK
GNGRTSPWEIYITLHPATAEDQDSQYVCFTLVLQV
PAEYPHEVPQISIRNPRGLSDEQIHTILQVLGHVA
KAGLGTAMLYELIEKGKEILTDNNIPHGQCVICLY
GFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELKA
QGQEQEQERQHATTKQKAVGVQCPVCREPLVYDLA
SLKAAPEPQQPMELYQPSAESLRQQEERKRLYQRQ
QERGGIIDLEAERNRYFISLQQPPAPAEPESAVDV
SKGSQPPSTLAAELSTSPAVQSTLPPPLPVATQHI
CEKIPGTRSNQQRLGETQKAMLDPPKPSRGPWRQP
ERRHPKGGECHAPKGTRDTQELPPPEGPLKEPMDL
KPEPHSQGVEGPPQEKGPGSWQGPPPRRTRDCVRW
ERSKGRTPGSSYPRLPRGQGAYRPGTRRESLGLES
KDGS- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of RNF25 expression in transfected 293T cell line (H00064320-T01) by RNF25 MaxPab polyclonal antibody.Lane 1: RNF25 transfected lysate(51.20 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to RNF25 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of RNF25 transfected lysate using anti-RNF25 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RNF25 MaxPab mouse polyclonal antibody (B01) (H00064320-B01).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
