Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28080 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF25 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: H1299.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RNF25 Polyclonal Antibody (Product # PA5-28080). Sample (30 µg of whole cell lysate). Lane A: H1299. 10% SDS PAGE. RNF25 Polyclonal Antibody (Product # PA5-28080) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RNF25 in methanol-fixed HeLa cells using a RNF25 polyclonal antibody (Product # PA5-28080) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RNF25 Polyclonal Antibody detects RNF25 protein at nucleus and cytosol on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. RNF25 Polyclonal Antibody (Product # PA5-28080) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.