14739-1-AP

antibody from Proteintech Group

Targeting: CYP27A1 CP27, CTX, CYP27

Provider product page for 14739-1-AP

Western blot

ELISA

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [31]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 14739-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14739-1-AP, RRID:AB_2089276
Product name: CYP27A1 antibody
Antibody type: Polyclonal
Description: CYP27A1 antibody (Cat. #14739-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CYP27A1 protein structure - 14739-1-AP shown in red.

Submitted references Diabetes Mellitus Facilitates Gallstone Formation Through CXCR2-NETs-Mediated Liver-Bile Barrier Damage.

Shi C, Feng S, Liu T, Meng Z, Zheng Y, Huang Z, Wang T, Zhang B, Xue D, Ma B, Meng X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Apr;13(24):e19500

A decrease in Flavonifractor plautii and its product, phytosphingosine, predisposes individuals with phlegm-dampness constitution to metabolic disorders.

Li L, Li T, Liang X, Zhu L, Fang Y, Dong L, Zheng Y, Xu X, Li M, Cai T, Zhao F, Xin M, Shao M, Guan Y, Liu M, Li F, Zhang C, Wang Q, Sun W, Zheng Y
Cell discovery 2025 Mar 17;11(1):25

NPC1L1 Drives Osteoporosis by Activating the C/EBPα/Cyp27a1/27-Hydroxycholesterol Axis: A Novel Therapeutic Target for Bone Loss.

Li B, Zhou W, Yu Y, Chen B, Lv Z, Zhang J, Zhang T, Sun S, Zhou L, Wang M
FASEB bioAdvances 2025 Jun;7(6):e70020

Effects of co-exposure to heat and ozone on lipid metabolism in the liver and adipose tissue of C57BL/6J male mice.

Jiang X, Li M, Li W, Guo Y, Zhang J, Ye L, Guo Z, Yang Y, Liu W, Chen L, Wang Q, Wu W, Dong G, Gui Z, Li D, Chen W, Chen S
Journal of hazardous materials 2025 Jun 5;489:137577

Yinchenhao Decoction Mitigates Cholestatic Liver Injury in Mice via Gut Microbiota Regulation and Activation of FXR-FGF15 Pathway.

Li W, Huang D, Luo Z, Zhou T, Jin Z
Pharmaceuticals (Basel, Switzerland) 2025 Jun 20;18(7)

Salvianolic acid B attenuates hypercholesterolemia via modulating the gut microbiota and bile acid metabolism.

Meng X, Chen Y, Zhao B, Liu Q, Zhao L, Zheng X, Feng W
European journal of pharmacology 2025 Jul 15;999:177701

Smilax china L. Extract Alleviates Metabolic-Associated Fatty Liver Disease by Regulating Gut Microbiota and Bile Acid Metabolism.

Cheng S, Li H, Sun Z, Xiong Y, Li J, Tian J, Shen Y, Shen L, Yang J, Yang Y, Liu D, Wei Q, Huang C, Ye X
Metabolites 2025 Dec 26;16(1)

Multiomics integration analysis identifies tumor cell-derived MIF as a therapeutic target and potentiates anti-PD-1 therapy in osteosarcoma.

Chen W, Liao Y, Yao H, Zou Y, Fang J, Zhang J, Guo Z, Tu J, Chen J, Huo Z, Wen L, Xie X
Journal for immunotherapy of cancer 2025 Aug 22;13(8)

Cu(OH)(2) nanopesticide induced liver dysfunction in mice by targeting lipoylated tricarboxylic acid cycle proteins via ferredoxin 1.

Ren Z, Ren M, Ling W, Ren D, Liang J, Cai Y, Wang X, Wang S, Duan Y, Ku T, Ning X, Sang N
Journal of hazardous materials 2025 Aug 15;494:138403

Multiomics combined analysis reveals protective effect of 7-O-α-L-rhamnopyranosyl-kaempferol-3-O-β-D-glucopyranoside on autoimmune hepatitis.

Xiao W, Li Z, Wang Y, Yongbo X, Li W, Li J, He M, Feng Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Apr;139:156460

Yellow tea polysaccharides protect against non-alcoholic fatty liver disease via regulation of gut microbiota and bile acid metabolism in mice.

Huang Y, Chen H, Chen J, Wu Q, Zhang W, Li D, Lu Y, Chen Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Oct;133:155919

Microbial Fermentation Enhances the Effect of Black Tea on Hyperlipidemia by Mediating Bile Acid Metabolism and Remodeling Intestinal Microbes.

Sun L, Wen L, Li Q, Chen R, Wen S, Lai X, Lai Z, Cao J, Zhang Z, Hao M, Cao F, Sun S
Nutrients 2024 Mar 28;16(7)

Dietary chenodeoxycholic acid attenuates high-fat diet-induced growth retardation, lipid accumulation and bile acid metabolism disorder in the liver of yellow catfish Pelteobagrus fulvidraco.

Zheng H, Xu YC, Zhao T, Luo Z, Zhang DG, Song CC, Yu AG, Tan X
The British journal of nutrition 2024 Mar 28;131(6):921-934

High level 27-HC impairs trophoblast cell invasion and migration via LXR in pre-eclampsia.

Tong H, Wang T, Chen R, Jin P, Jiang Y, Zhang X, Qi H
Experimental cell research 2024 Apr 1;437(1):113979

Chronic corticosterone exposure disrupts hepatic and intestinal bile acid metabolism in chicken.

Wu L, Liu X, Zhang A, Chen H, Zhao R, Jia Y
Frontiers in veterinary science 2023;10:1147024

Targeting mTOR/YY1 signaling pathway by quercetin through CYP7A1-mediated cholesterol-to-bile acids conversion alleviated type 2 diabetes mellitus induced hepatic lipid accumulation.

Yang T, Wang Y, Cao X, Peng Y, Huang J, Chen L, Pang J, Jiang Z, Qian S, Liu Y, Ying C, Wang T, Zhang F, Lu Q, Yin X
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 May;113:154703

Supplemental Clostridium butyricum modulates lipid metabolism by reshaping the gut microbiota composition and bile acid profile in IUGR suckling piglets.

Zhang X, Yun Y, Lai Z, Ji S, Yu G, Xie Z, Zhang H, Zhong X, Wang T, Zhang L
Journal of animal science and biotechnology 2023 Mar 13;14(1):36

ANO1 Reprograms Cholesterol Metabolism and the Tumor Microenvironment to Promote Cancer Metastasis.

Deng CM, Zhang GG, Liu QW, Xu JJ, Liu ZC, Yang J, Xu TY, Li ZG, Zhang F, Li B
Cancer research 2023 Jun 2;83(11):1851-1865

Lactobacillus gasseri LA39 promotes hepatic primary bile acid biosynthesis and intestinal secondary bile acid biotransformation.

Hu J, Hou Q, Zheng W, Yang T, Yan X
Journal of Zhejiang University. Science. B 2023 Aug 15;24(8):734-748

FXR Signaling-Mediated Bile Acid Metabolism Is Critical for Alleviation of Cholesterol Gallstones by Lactobacillus Strains.

Ye X, Huang D, Dong Z, Wang X, Ning M, Xia J, Shen S, Wu S, Shi Y, Wang J, Wan X
Microbiology spectrum 2022 Oct 26;10(5):e0051822

Oryzanol Attenuates High Fat and Cholesterol Diet-Induced Hyperlipidemia by Regulating the Gut Microbiome and Amino Acid Metabolism.

Yan S, Chen J, Zhu L, Guo T, Qin D, Hu Z, Han S, Zhou Y, Akan OD, Wang J, Luo F, Lin Q
Journal of agricultural and food chemistry 2022 Jun 1;70(21):6429-6443

Bird's eye view analysis of in situ cholesterol metabolic pathways in breast cancer patients and its clinicopathological significance in their subtypes.

Kosaka S, Miyashita M, McNamala K, Nomura M, Shima H, Kawai M, Sato I, Harada-Shoji N, Ishida T, Choi MH, Sasano H
The Journal of steroid biochemistry and molecular biology 2022 Jul;221:106103

Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.

Fu Y, Feng H, Ding X, Meng QH, Zhang SR, Li J, Chao Y, Ji TT, Bi YH, Zhang WW, Chen Q, Zhang YH, Feng YL, Bian HM
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jul;101:154120

Chemical profile of Swertia mussotii Franch and its potential targets against liver fibrosis revealed by cross-platform metabolomics.

Zhang Y, Zhou Q, Ding X, Ma J, Tan G
Journal of ethnopharmacology 2021 Jun 28;274:114051

Dolomiaea souliei ethyl acetate extract protected against α-naphthylisothiocyanate-induced acute intrahepatic cholestasis through regulation of farnesoid x receptor-mediated bile acid metabolism.

Meng F, Zong W, Wei X, Tao Y, Wang G, Liao Z, Chen M
Phytomedicine : international journal of phytotherapy and phytopharmacology 2021 Jul;87:153588

Dehydrodiconiferyl alcohol, a lignan from Herpetospermum pedunculosum, alleviates cholestasis by activating pathways associated with the farnesoid X receptor.

Wei X, Ma Y, Dong Z, Wang G, Lan X, Liao Z, Chen M
Phytomedicine : international journal of phytotherapy and phytopharmacology 2021 Jan;80:153378

Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.

Teng S, Li YE, Yang M, Qi R, Huang Y, Wang Q, Zhang Y, Chen S, Li S, Lin K, Cao Y, Ji Q, Gu Q, Cheng Y, Chang Z, Guo W, Wang P, Garcia-Bassets I, Lu ZJ, Wang D
Cell research 2020 Jan;30(1):34-49

Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia.

Mistry HD, Kurlak LO, Mansour YT, Zurkinden L, Mohaupt MG, Escher G
Journal of lipid research 2017 Jun;58(6):1186-1195

Maternal Betaine Supplementation throughout Gestation and Lactation Modifies Hepatic Cholesterol Metabolic Genes in Weaning Piglets via AMPK/LXR-Mediated Pathway and Histone Modification.

Cai D, Yuan M, Liu H, Pan S, Ma W, Hong J, Zhao R
Nutrients 2016 Oct 18;8(10)

Dysregulated bile acid synthesis, metabolism and excretion in a high fat-cholesterol diet-induced fibrotic steatohepatitis in rats.

Jia X, Naito H, Yetti H, Tamada H, Kitamori K, Hayashi Y, Wang D, Yanagiba Y, Wang J, Ikeda K, Yamori Y, Nakajima T
Digestive diseases and sciences 2013 Aug;58(8):2212-22

Cholesterol-enriched diet causes age-related macular degeneration-like pathology in rabbit retina.

Dasari B, Prasanthi JR, Marwarha G, Singh BB, Ghribi O
BMC ophthalmology 2011 Aug 18;11:22

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14739-1-AP(CYP27A1 antibody) at dilution of 1:400
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human liver using 14739-1-AP(CYP27A1 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The CYP27A1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CYP27A1. This antibody recognizes human,mouse,rat antigen. The CYP27A1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 14739-1-AP.pdf

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