14739-1-AP

antibody from Proteintech Group

Targeting: CYP27A1 CP27, CTX, CYP27

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14739-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14739-1-AP, RRID:AB_2089276

Product name

CYP27A1 antibody

Antibody type

Polyclonal

Description

CYP27A1 antibody (Cat. #14739-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Microbial Fermentation Enhances the Effect of Black Tea on Hyperlipidemia by Mediating Bile Acid Metabolism and Remodeling Intestinal Microbes.

Sun L, Wen L, Li Q, Chen R, Wen S, Lai X, Lai Z, Cao J, Zhang Z, Hao M, Cao F, Sun S
Nutrients 2024 Mar 28;16(7)

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Dietary chenodeoxycholic acid attenuates high-fat diet-induced growth retardation, lipid accumulation and bile acid metabolism disorder in the liver of yellow catfish Pelteobagrus fulvidraco.

Zheng H, Xu YC, Zhao T, Luo Z, Zhang DG, Song CC, Yu AG, Tan X
The British journal of nutrition 2024 Mar 28;131(6):921-934

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High level 27-HC impairs trophoblast cell invasion and migration via LXR in pre-eclampsia.

Tong H, Wang T, Chen R, Jin P, Jiang Y, Zhang X, Qi H
Experimental cell research 2024 Apr 1;437(1):113979

---

Chronic corticosterone exposure disrupts hepatic and intestinal bile acid metabolism in chicken.

Wu L, Liu X, Zhang A, Chen H, Zhao R, Jia Y
Frontiers in veterinary science 2023;10:1147024

---

Targeting mTOR/YY1 signaling pathway by quercetin through CYP7A1-mediated cholesterol-to-bile acids conversion alleviated type 2 diabetes mellitus induced hepatic lipid accumulation.

Yang T, Wang Y, Cao X, Peng Y, Huang J, Chen L, Pang J, Jiang Z, Qian S, Liu Y, Ying C, Wang T, Zhang F, Lu Q, Yin X
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 May;113:154703

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Supplemental Clostridium butyricum modulates lipid metabolism by reshaping the gut microbiota composition and bile acid profile in IUGR suckling piglets.

Zhang X, Yun Y, Lai Z, Ji S, Yu G, Xie Z, Zhang H, Zhong X, Wang T, Zhang L
Journal of animal science and biotechnology 2023 Mar 13;14(1):36

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Lactobacillus gasseri LA39 promotes hepatic primary bile acid biosynthesis and intestinal secondary bile acid biotransformation.

Hu J, Hou Q, Zheng W, Yang T, Yan X
Journal of Zhejiang University. Science. B 2023 Aug 15;24(8):734-748

---

FXR Signaling-Mediated Bile Acid Metabolism Is Critical for Alleviation of Cholesterol Gallstones by Lactobacillus Strains.

Ye X, Huang D, Dong Z, Wang X, Ning M, Xia J, Shen S, Wu S, Shi Y, Wang J, Wan X
Microbiology spectrum 2022 Oct 26;10(5):e0051822

---

Oryzanol Attenuates High Fat and Cholesterol Diet-Induced Hyperlipidemia by Regulating the Gut Microbiome and Amino Acid Metabolism.

Yan S, Chen J, Zhu L, Guo T, Qin D, Hu Z, Han S, Zhou Y, Akan OD, Wang J, Luo F, Lin Q
Journal of agricultural and food chemistry 2022 Jun 1;70(21):6429-6443

---

Bird's eye view analysis of in situ cholesterol metabolic pathways in breast cancer patients and its clinicopathological significance in their subtypes.

Kosaka S, Miyashita M, McNamala K, Nomura M, Shima H, Kawai M, Sato I, Harada-Shoji N, Ishida T, Choi MH, Sasano H
The Journal of steroid biochemistry and molecular biology 2022 Jul;221:106103

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Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.

Fu Y, Feng H, Ding X, Meng QH, Zhang SR, Li J, Chao Y, Ji TT, Bi YH, Zhang WW, Chen Q, Zhang YH, Feng YL, Bian HM
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jul;101:154120

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Chemical profile of Swertia mussotii Franch and its potential targets against liver fibrosis revealed by cross-platform metabolomics.

Zhang Y, Zhou Q, Ding X, Ma J, Tan G
Journal of ethnopharmacology 2021 Jun 28;274:114051

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Dolomiaea souliei ethyl acetate extract protected against α-naphthylisothiocyanate-induced acute intrahepatic cholestasis through regulation of farnesoid x receptor-mediated bile acid metabolism.

Meng F, Zong W, Wei X, Tao Y, Wang G, Liao Z, Chen M
Phytomedicine : international journal of phytotherapy and phytopharmacology 2021 Jul;87:153588

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Dehydrodiconiferyl alcohol, a lignan from Herpetospermum pedunculosum, alleviates cholestasis by activating pathways associated with the farnesoid X receptor.

Wei X, Ma Y, Dong Z, Wang G, Lan X, Liao Z, Chen M
Phytomedicine : international journal of phytotherapy and phytopharmacology 2021 Jan;80:153378

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Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.

Teng S, Li YE, Yang M, Qi R, Huang Y, Wang Q, Zhang Y, Chen S, Li S, Lin K, Cao Y, Ji Q, Gu Q, Cheng Y, Chang Z, Guo W, Wang P, Garcia-Bassets I, Lu ZJ, Wang D
Cell research 2020 Jan;30(1):34-49

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Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia.

Mistry HD, Kurlak LO, Mansour YT, Zurkinden L, Mohaupt MG, Escher G
Journal of lipid research 2017 Jun;58(6):1186-1195

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Maternal Betaine Supplementation throughout Gestation and Lactation Modifies Hepatic Cholesterol Metabolic Genes in Weaning Piglets via AMPK/LXR-Mediated Pathway and Histone Modification.

Cai D, Yuan M, Liu H, Pan S, Ma W, Hong J, Zhao R
Nutrients 2016 Oct 18;8(10)

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Dysregulated bile acid synthesis, metabolism and excretion in a high fat-cholesterol diet-induced fibrotic steatohepatitis in rats.

Jia X, Naito H, Yetti H, Tamada H, Kitamori K, Hayashi Y, Wang D, Yanagiba Y, Wang J, Ikeda K, Yamori Y, Nakajima T
Digestive diseases and sciences 2013 Aug;58(8):2212-22

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Cholesterol-enriched diet causes age-related macular degeneration-like pathology in rabbit retina.

Dasari B, Prasanthi JR, Marwarha G, Singh BB, Ghribi O
BMC ophthalmology 2011 Aug 18;11:22

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14739-1-AP(CYP27A1 antibody) at dilution of 1:400

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 14739-1-AP(CYP27A1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The CYP27A1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CYP27A1. This antibody recognizes human,mouse,rat antigen. The CYP27A1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

14739-1-AP.pdf

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