MA5-34810

antibody from Invitrogen Antibodies

Targeting: CYP27A1 CP27, CTX, CYP27

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34810

Provider

Invitrogen Antibodies

Product name

CYP27A1 Recombinant Rabbit Monoclonal Antibody (JG40-36)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Rat heart tissue, human liver tissue, human kidney tissue, human small intestine tissue, HepG2, LOVO.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG40-36

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CYP27A1 in Positive control: Lane 1: Rat heart, Lane 2: Human liver, Lane 2: Human kidney. Samples were incubated with CYP27A1 monoclonal antibody (Product # MA5-34810), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CYP27A1 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with CYP27A1 monoclonal antibody (Product # MA5-34810), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CYP27A1 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with CYP27A1 monoclonal antibody (Product # MA5-34810), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CYP27A1 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with CYP27A1 monoclonal antibody (Product # MA5-34810), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CYP27A1 in paraffin-embedded human small intestine tissue. Samples were incubated with CYP27A1 monoclonal antibody (Product # MA5-34810), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CYP27A1 in paraffin-embedded human liver cancer tissue. Samples were incubated with CYP27A1 monoclonal antibody (Product # MA5-34810), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CYP27A1 in paraffin-embedded human kidney tissue. Samples were incubated with CYP27A1 monoclonal antibody (Product # MA5-34810), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of CYP27A1 in HepG2 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with CYP27A1 monoclonal antibody (Product # MA5-34810) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.