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- Antibody Data
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- Immunocytochemistry [1]
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- Product number
- RQ5879 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CYP27A1 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific CYP27A1 antibody is suitable for use in Western blot/Immunofluorescence/Direct ELISA applications with human, mouse and rat, monkey samples.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the CYP27A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) placenta, 2) HepG2, 3) Caco-2, 4) SW620, 5) SGC-7901, 6) HEK293, 7) monkey liver, 8) human U-2 OS, 9) rat liver, 10) rat heart, 11) mouse liver, 12) mouse heart and 13) mouse NIH 3T3 lysate with CYP27A1 antibody. Expected molecular weight: 60-65 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A431 cells with CYP27A1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
