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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-29903 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP27A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, mouse liver. Predicted reactivity: Pig (80%), Rabbit (82%), Rhesus Monkey (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.78 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CYP27A1 Polyclonal Antibody (Product # PA5-29903). Sample (30 µg of whole cell lysate). Lane A: Hep G2 . 7.5% SDS PAGE. CYP27A1 Polyclonal Antibody . CYP27A1 Polyclonal Antibody (Product # PA5-29903) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CYP27A1 Polyclonal Antibody (Product # PA5-29903). Sample (50 µg of whole cell lysate). Lane A: mouse Liver . 7.5% SDS PAGE. CYP27A1 Polyclonal Antibody (Product # PA5-29903) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded hela xenograft, using CYP27A1 Polyclonal Antibody (Product # PA5-29903) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
