102-PA22

antibody from ReliaTech GmbH

Targeting: FLT4 PCL, VEGFR-3, VEGFR3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

102-PA22

Provider

ReliaTech GmbH

Product name

VEGFR-3/FLT-4

Antibody type

Polyclonal

Antigen

recombinant human soluble FLT-4 (D1-7)

Description

antibody Protein-A purified from serum

Reactivity

Human

Host

Rabbit

Antigen sequence

DPSGYSMTPPTLNITEDSYVIDTGDSLSISCRGQH
PLEWTWPGAQEVLTTGGKDSEDTRVVHDCEGTEAR
PYCKVLLLAQTHANNTGSYHCYYKYIKARIEGTTA
ASTYVFVRDFKHPFINKPDTLLVNRKDSMWVPCLV
SIPGLNITLRSQSSALHPDGQEVLWDDRRGMRVPT
QLLRDALYLQCETTWGDQNFLSNLFVVHITGNELY
DIQLYPKKSMELLVGEKLVLNCTVWAEFDSGVTFD
WDYPGKQAERAKWVPERRSQQTHTELSSILTIHNV
SQNDLGPYVCEANNGIQRFRESTEVIVHEKPFISV
EWLKGPVLEATAGDELVKLPVKLAAYPPPEFQWYK
DRKAVTGRHNPHALVLKEVTEASAGVYTLALWNSA
AGLRQNISLELVVNVPPHIHEKEASSPSIYSRHSR
QTLTCTAYGVPQPLSVQWHWRPWTPCKTFAQRSLR
RRQQRDGMPQCRDWKEVTTQDAVNPIESLDSWTEF
VEGKNKTVSKLVIQDANVSAMYKCVVVNKVGQDER
LIYFYVTTIPDGFSIESEPSEDPLEGQSVRLSCRA
DNYTYEHLRWYRLNLSTLHDAQGNPLLLDCKNVHL
FATPLEANLEEAEPGARHATLSLNIPRVAPEDEGD
YVCEVQDRRSQDKHCHKKYLSVQALEAPRLTQNLT
DLLVNVSDSLEMRCPVAGAHVPSIVWYKDERLLEK
ESGIDLADSNQRLSIQRVREEDAGRYLCSVCNAKG
CVNSSASVAVEGSEDKGSMEHHHHHH


Antibody clone number

Rabbit IG

Vial size

200 µl

Storage

Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.

Handling

Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.

References

Submitted references

Endothelial EphB4 maintains vascular integrity and transport function in adult heart.

Luxán G, Stewen J, Díaz N, Kato K, Maney SK, Aravamudhan A, Berkenfeld F, Nagelmann N, Drexler HC, Zeuschner D, Faber C, Schillers H, Hermann S, Wiseman J, Vaquerizas JM, Pitulescu ME, Adams RH
eLife 2019 Nov 29;8

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Functional Importance of a Proteoglycan Coreceptor in Pathologic LymphangiogenesisNovelty and Significance

Johns S, Yin X, Jeltsch M, Bishop J, Schuksz M, El Ghazal R, Wilcox-Adelman S, Alitalo K, Fuster M
Circulation Research 2016 July;119(2):210-221

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Lymphatic Endothelial Heparan Sulfate Deficiency Results in Altered Growth Responses to Vascular Endothelial Growth Factor-C (VEGF-C)

Yin X, Johns S, Lawrence R, Xu D, Reddi K, Bishop J, Varner J, Fuster M
Journal of Biological Chemistry 2011 April;286(17):14952-14962

Western blot

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

Western analysis of recombinant human soluble VEGFR3/FLT-4 using a rabbit anti-human FLT-4 antibody [Cat# 102-PA22]. Lane ½: purified proteins; Lane 4/5: insect cells supernatant.

Immunocytochemistry

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

Immunofluorescence staining of human VEGFR-3/FLT-4 (red) in hFlt4-transfected MG63 cells. Monoclonal mouse anti-human FLT-4 #9D9 [Cat# 101-M36] and polyclonal rabbit anti-human FLT-4 (D) [Cat# 102-PA22]: A and C are negative control with secondary antibody only; B (mAb) and D (pAb) with specific antibodies against human FLT-4. The experiment was performed by the research group of Dr. Wolfgang Holnthoner, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, A-1200 Vienna, Austria

Sample type

FLT-4 transfected MG63 cells

Immunohistochemistry

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

IHC with human spleen tissue sections. The experiment was performed by Prof. Dr. Birte Steiniger, Institute of Anatomy and Cell Biology Robert-Koch-Str. 8, D-35037 Marburg, Germany

Sample type

Human Spleen

Flow cytometry

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).