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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- 101-M36 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- VEGFR-3/FLT-4
- Antibody type
- Monoclonal
- Antigen
- recombinant human soluble FLT-4 (D1-7)
- Description
- antibody purified from hybridoma supernatant
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
DPSGYSMTPPTLNITEDSYVIDTGDSLSISCRGQH
PLEWTWPGAQEVLTTGGKDSEDTRVVHDCEGTEAR
PYCKVLLLAQTHANNTGSYHCYYKYIKARIEGTTA
ASTYVFVRDFKHPFINKPDTLLVNRKDSMWVPCLV
SIPGLNITLRSQSSALHPDGQEVLWDDRRGMRVPT
QLLRDALYLQCETTWGDQNFLSNLFVVHITGNELY
DIQLYPKKSMELLVGEKLVLNCTVWAEFDSGVTFD
WDYPGKQAERAKWVPERRSQQTHTELSSILTIHNV
SQNDLGPYVCEANNGIQRFRESTEVIVHEKPFISV
EWLKGPVLEATAGDELVKLPVKLAAYPPPEFQWYK
DRKAVTGRHNPHALVLKEVTEASAGVYTLALWNSA
AGLRQNISLELVVNVPPHIHEKEASSPSIYSRHSR
QTLTCTAYGVPQPLSVQWHWRPWTPCKTFAQRSLR
RRQQRDGMPQCRDWKEVTTQDAVNPIESLDSWTEF
VEGKNKTVSKLVIQDANVSAMYKCVVVNKVGQDER
LIYFYVTTIPDGFSIESEPSEDPLEGQSVRLSCRA
DNYTYEHLRWYRLNLSTLHDAQGNPLLLDCKNVHL
FATPLEANLEEAEPGARHATLSLNIPRVAPEDEGD
YVCEVQDRRSQDKHCHKKYLSVQALEAPRLTQNLT
DLLVNVSDSLEMRCPVAGAHVPSIVWYKDERLLEK
ESGIDLADSNQRLSIQRVREEDAGRYLCSVCNAKG
CVNSSASVAVEGSEDKGSMEHHHHHH- Isotype
- IgG
- Antibody clone number
- (#9D9)
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references Absence of lymphatic vessels in term placenta.
Molecular and Cellular Effects of In Vitro Shockwave Treatment on Lymphatic Endothelial Cells
Effective suppression of vascular network formation by combination of antibodies blocking VEGFR ligand binding and receptor dimerization.
Similarities and differences of human and experimental mouse lymphangiomas
Immunodetection and quantification of vascular endothelial growth factor receptor-3 in human malignant tumor tissues
Becker J, Tchagou Tchangou GE, Schmidt S, Zelent C, Kahl F, Wilting J
BMC pregnancy and childbirth 2020 Jun 29;20(1):380
BMC pregnancy and childbirth 2020 Jun 29;20(1):380
Molecular and Cellular Effects of In Vitro Shockwave Treatment on Lymphatic Endothelial Cells
Rohringer S, Holnthoner W, Hackl M, Weihs A, Rünzler D, Skalicky S, Karbiener M, Scheideler M, Pröll J, Gabriel C, Schweighofer B, Gröger M, Spittler A, Grillari J, Redl H, Dulak J
PLoS ONE 2014 December;9(12)
PLoS ONE 2014 December;9(12)
Effective suppression of vascular network formation by combination of antibodies blocking VEGFR ligand binding and receptor dimerization.
Tvorogov D, Anisimov A, Zheng W, Leppänen VM, Tammela T, Laurinavicius S, Holnthoner W, Heloterä H, Holopainen T, Jeltsch M, Kalkkinen N, Lankinen H, Ojala PM, Alitalo K
Cancer cell 2010 Dec 14;18(6):630-40
Cancer cell 2010 Dec 14;18(6):630-40
Similarities and differences of human and experimental mouse lymphangiomas
Kasten P, Schnöink G, Bergmann A, Papoutsi M, Buttler K, Rössler J, Weich H, Wilting J
Developmental Dynamics 2007 October;236(10):2952-2961
Developmental Dynamics 2007 October;236(10):2952-2961
Immunodetection and quantification of vascular endothelial growth factor receptor-3 in human malignant tumor tissues
Bando H, Brokelmann M, Toi M, Alitalo K, Sleeman J, Sipos B, Gr�ne H, Weich H
International Journal of Cancer 2004 August;111(2):184-191
International Journal of Cancer 2004 August;111(2):184-191
No comments: Submit comment
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western analysis of recombinant human soluble VEGFR-3/FLT-4.
- Sample type
- Purified recombinant protein
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining of human VEGFR-3/FLT-4 (red) in hFlt4-transfected MG63 cells. Monoclonal mouse anti-human FLT-4 #9D9 [Cat# 101-M36] and polyclonal rabbit anti-human FLT-4 (D) [Cat# 102-PA22]: A and C are negative control with secondary antibody only; B (mAb) and D (pAb) with specific antibodies against human FLT-4. The experiment was performed by the research group of Dr. Wolfgang Holnthoner, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, A-1200 Vienna, Austria
- Sample type
- FLT-4 transfected MG63 cells
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with HDMVEC and HUVEC.
- Sample type
- HDMVEC and HUVEC
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).
