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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunohistochemistry [1]
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- Product number
- A01276-3 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-VEGF Receptor 3/FLT4 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for VEGF R3/FLT4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IHC-F, ICC/IF, FCM, Direct ELISA. Reactive species: Human;Mouse;Rat. VEGF R3/FLT4 information: Subcellular Localization: Cell membrane; Ligand-mediated autophosphorylation leads to rapid internalization; Tissue Specificity: Detected in endothelial cells (at protein level). Widely expressed. Detected in fetal spleen, lung and brain. Detected in adult liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Expression levels of VEGF-C and VEGFR-3 in renal cell carcinoma and their association with lymph node metastasis.
Expression of angiopoietin-2 and vascular endothelial growth factor receptor-3 correlates with lymphangiogenesis and angiogenesis and affects survival of oral squamous cell carcinoma.
Antitumor activity of endogenous mFlt4 displayed on a T4 phage nanoparticle surface.
Expression of cyclooxygenase-2 and vascular endothelial growth factor-C correlates with lymphangiogenesis and lymphatic invasion in human gastric cancer.
Li X, Song D, Liu H, Wang Z, Ma G, Yu M, Zhang Y, Zeng Y
Experimental and therapeutic medicine 2021 Jun;21(6):554
Experimental and therapeutic medicine 2021 Jun;21(6):554
Expression of angiopoietin-2 and vascular endothelial growth factor receptor-3 correlates with lymphangiogenesis and angiogenesis and affects survival of oral squamous cell carcinoma.
Li C, Fan J, Song X, Zhang B, Chen Y, Li C, Mi K, Ma H, Song Y, Tao X, Li G
PloS one 2013;8(9):e75388
PloS one 2013;8(9):e75388
Antitumor activity of endogenous mFlt4 displayed on a T4 phage nanoparticle surface.
Ren SX, Ren ZJ, Zhao MY, Wang XB, Zuo SG, Yu F
Acta pharmacologica Sinica 2009 May;30(5):637-45
Acta pharmacologica Sinica 2009 May;30(5):637-45
Expression of cyclooxygenase-2 and vascular endothelial growth factor-C correlates with lymphangiogenesis and lymphatic invasion in human gastric cancer.
Da MX, Wu XT, Wang J, Guo TK, Zhao ZG, Luo T, Zhang MM, Qian K
Archives of medical research 2008 Jan;39(1):92-9
Archives of medical research 2008 Jan;39(1):92-9
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-3). VEGF Receptor 3 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VEGF Receptor 3 Antibody (A01276-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
