MA5-15651

antibody from Invitrogen Antibodies

Targeting: FLT4 PCL, VEGFR-3, VEGFR3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15651

Provider

Invitrogen Antibodies

Product name

VEGF Receptor 3 Monoclonal Antibody (4H4)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15651 targets FLT4 in WB applications and shows reactivity with Human samples.

Antibody clone number

4H4

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FLT4 using FLT4 monoclonal antibody (Product # MA5-15651) in FLT4 (AA: 25-330) human IgG Fc transfected HEK293 cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Vascular endothelial growth factor receptor 3 was performed using 70% confluent log phase HMEC-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with VEGF Receptor 3 Monoclonal Antibody (4H4) (Product # MA5-15651) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained withRhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoplasmic localization. Panel e represents Low expression in HUVEC. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification in EVOS™ M7000 Imaging System (Product # AMF7000) and externally deconvoluted (D.Sage et al. / Methods 115 (2017) 28-41).