- PA5-21676 - Invitrogen Antibodies BCL2L1 antibody

Submitted references Long Stress Induced Non-Coding Transcripts 5 (LSINCT5) Promotes Hepatocellular Carcinoma Progression Through Interaction with High-Mobility Group AT-hook 2 and MiR-4516.

Li O, Li Z, Tang Q, Li Y, Yuan S, Shen Y, Zhang Z, Li N, Chu K, Lei G
Medical science monitor : international medical journal of experimental and clinical research 2018 Nov 25;24:8510-8523

Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats.

Wu Q, Dai Q, Jiang L, Wang Y, Yang T, Miao J, Wang J, Han Y
Molecular medicine reports 2017 Aug;16(2):1578-1583

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BCL2L1 using 30 µg of HCT116 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a BCL2L1 polyclonal antibody (Product # PA5-21676) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Bcl-X was achieved by transfecting A549 cells with Bcl-X (BCL2L1) specific validated siRNA (Silencer® select Product # s1920). Western blot analysis (Fig. a) was performed using whole cell extracts from the Bcl-X knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-Bcl-X Polyclonal Antibody (Product # PA5-21676, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Bcl-X.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Bcl-X Polyclonal Antibody (Product # PA5-21676). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 12% SDS PAGE. Bcl-X Polyclonal Antibody (Product # PA5-21676) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Bcl-X Polyclonal Antibody (Product # PA5-21676). HepG2 and mitochondria extracts (30 µg) were separated by SDS-PAGE, and the membrane was blotted with Bcl-X Polyclonal Antibody (Product # PA5-21676) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Bcl-X Polyclonal Antibody detects BCL2L1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.12% SDS-PAGE. Bcl-X Polyclonal Antibody (Product # PA5-21676) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Bcl-X Polyclonal Antibody (Product # PA5-21676). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-X Polyclonal Antibody (Product # PA5-21676) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockout of BCL2L1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR962685_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of BCL2L1 was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa CAS9 (Lane 2) and HeLa BCL2L1 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-Bcl-X Polyclonal Antibody (Product # PA5-21676, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to BCL2L1. An uncharacterized band was observed at ~60 kda.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of BCL2L1 in methanol-fixed A549 cells using a BCL2L1 polyclonal antibody (Product # PA5-21676) at a 1:500 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of Bcl-X was performed in SK-N-SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Bcl-X Polyclonal Antibody (Product # PA5-21676) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of methanol-fixed A549, using BCL-x antibody (Product # PA5-21676) at 1:500 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of Bcl-X was performed in paraffin-embedded human breast carcinoma tissue using Bcl-X Polyclonal Antibody (Product # PA5-21676) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCL2L1 (Product # PA5-21676) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-21676

Antibody data