R35438

antibody from NSJ Bioreagents

Targeting: NHEJ1 Cernunnos, FLJ12610, XLF

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R35438

Provider

NSJ Bioreagents

Product name

XLF Antibody / XRCC4-like factor

Antibody type

Polyclonal

Description

This highly specific XLF antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry ELISA applications with human samples.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide

Storage

Aliquot and store the XLF antibody at -20oC.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human thyroid lysate with XLF antibody at 0.1ug/ml. Predicted molecular weight: ~33kDa, routinely observed at ~37kDa. (Ref 1)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human thyroid lysate with XLF antibody at 0.1ug/ml. Predicted molecular weight: ~33kDa, routinely observed at ~37kDa. (Ref 1)

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC staining of fixed and permeabilized human U-2 OS cells with XLF antibody (green) at 10ug/ml, DAPI nuclear stain (blue) and Phalloidin (red).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC staining of fixed and permeabilized human HepG2 cells with XLF antibody (green) at 10ug/ml, DAPI nuclear stain (blue) and Phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placenta with XLF antibody at 4ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS testing of fixed and permeabilized human HepG2 cells with XLF antibody (blue) at 10ug/ml and naive goat Ig (black).