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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA502176S - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NHEJ1 Monoclonal Antibody (OTI1G5), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1G5
- Vial size
- 30 µL
- Concentration
- 1.6 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NHEJ1 (RC203393, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1. Positive lysates LY403031 (100 µg) and LC403031 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-NHEJ1 monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and NHEJ1-Knockout HeLa cells (KO, Cat#LC831412) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1 monoclonal antibody TA502176(1:200). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-NHEJ1 mouse monoclonal antibody (TA502176) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NHEJ1(RC203393).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC203393 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NHEJ1 antibody (TA502176), and then analyzed by flow cytometry.
