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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-27102 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- XLF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, HepG2, Raji. Predicted reactivity: Rhesus Monkey (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- XLF Polyclonal Antibody detects XLF protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: XLF protein stained by XLF Polyclonal Antibody (Product # PA5-27102) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- XLF Polyclonal Antibody immunoprecipitates XLF protein in IP experiments. IP samples: Jurkat whole cell extract. A. Control with 3 µg of preimmune Rabbit IgG. B. Immunoprecipitation of XLF protein by 3 µg XLF Polyclonal Antibody (Product # PA5-27102). 10 % SDS-PAGE. The immunoprecipitated XLF protein was detected by XLF Polyclonal Antibody (Product # PA5-27102) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- XLF Polyclonal Antibody detects XLF protein on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded ovarian carcinoma. XLF Polyclonal Antibody (Product # PA5-27102) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- XLF Polyclonal Antibody immunoprecipitates XLF protein in IP experiments. IP samples: Jurkat whole cell extract. A. Control with 3 µg of preimmune Rabbit IgG. B. Immunoprecipitation of XLF protein by 3 µg XLF Polyclonal Antibody (Product # PA5-27102). 10 % SDS-PAGE. The immunoprecipitated XLF protein was detected by XLF Polyclonal Antibody (Product # PA5-27102) diluted at 1:1,000.
