AP31820PU-N

antibody from Acris Antibodies GmbH

Targeting: MPZ CMT1, CMT1B, CMT2I, CMT2J, HMSNIB, P0

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AP31820PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP31820PU-N, RRID:AB_11144978

Product name

anti MPZ

Antibody type

Polyclonal

Antigen

Two antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (NP_032649) and Human (NP_000521) gene products.Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of both of these affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Reactivity

Human, Mouse

Host

Chicken/Avian

Vial size

0.2 mg

Concentration

0.2 mg/ml. The concentrations of each of the two antibodies was 0.1 mg/ml (based on Bradford assay readings using BSA as a standard).

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

In the Left panel (a tissue section through an adult sciatic nerve), Po (green staining) can be seen in the myelin and Schwann cell processes surrounding the nodes of Ranvier. In this photomicrograph, Rabbit antibodies against LAMP (lysozome-associated membrane glycoprotein) (red staining) serves as the counterstain, and DAPI (blue staining) allows visualization of nuclei. In the Right panel (a lower power tissue section through an adult sciatic nerve), Po (brown staining) can be seen in all of the myelinating Schwann cells.