TA324637

antibody from OriGene

Targeting: ATG9A APG9L1, FLJ22169

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324637

Provider

OriGene

Product name

Rabbit polyclonal ATG9A Antibody (C-term)

Antibody type

Polyclonal

Description

Rabbit polyclonal ATG9A Antibody (C-term)

Host

Rabbit

Conjugate

Unconjugated

Epitope

ATG9A

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

0.4 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of anti-Autophagy APG9L1 Antibody (C-term) (Cat.#TA324637) in A2058 and A375 cell line lysates (35ug/lane). APG9L1(arrow) was detected using the purified Pab.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of U251 cells stained with ATG9A (C-term) antibody. U251 cells were treated with Chloroquine , then incubated with TA324637 ATG9A (C-term) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . ATG9A immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Validation comment

IHC