ARG54825

antibody from Arigo

Targeting: ATG9A APG9L1, FLJ22169

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG54825

Provider

Arigo

Product name

anti-ATG9A antibody

Antibody type

Polyclonal

Antigen

KLH-conjugated synthetic peptide around aa. 717-746 (C-terminus) of Human ATG9A.

Description

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

200 µl

Storage

For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 35 µg of A2058 and A375 cell line lysates stained with ARG54825 anti-ATG9A antibody.

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: U251 cells stained with ARG54825 anti-ATG9A antibody (green) at 1:100 dilution, 2 h at room temperature. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ARG54825 anti-ATG9A antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). ATG9A immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells. .

Immunohistochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunohistochemistry: formalin-fixed and paraffin-embedded human cancer tissue stained with ARG54825 anti-ATG9A antibody.