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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [8]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-85515 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG9A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Keep as concentrated solution.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATG9A in whole cell extract using ATG9A polyclonal antibody (Product # PA5-85515) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATG9A in A) PC-12 whole cell lysate, B) rat-2 whole cell lysate using ATG9A polyclonal antibody (Product # PA5-85515) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATG9A in A) BCL-1 whole cell lysate using ATG9A polyclonal antibody (Product # PA5-85515) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATG9A in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa whole cell lysate using ATG9A polyclonal antibody (Product # PA5-85515) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ATG9A Polyclonal Antibody (Product # PA5-85515). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9A Polyclonal Antibody (Product # PA5-85515) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATG9A Polyclonal Antibody detects ATG9A protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9A Polyclonal Antibody (Product # PA5-85515) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATG9A Polyclonal Antibody detects ATG9A protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract.7.5% SDS-PAGE. ATG9A Polyclonal Antibody (Product # PA5-85515) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATG9A Polyclonal Antibody detects ATG9A protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat-2 whole cell lysate/extract.7.5% SDS-PAGE. ATG9A Polyclonal Antibody (Product # PA5-85515) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATG9A Polyclonal Antibody detects ATG9A protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 100% MeOH for 5 min. Green: ATG9A protein stained by ATG9A Polyclonal Antibody (Product # PA5-85515) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATG9A Polyclonal Antibody detects ATG9A protein on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. ATG9A Polyclonal Antibody (Product # PA5-85515) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of ATG9A in HeLa whole cell extract, A) control with preimmune rabbit IgG, B) immunoprecipitation of ATG9A protein with ATG9A polyclonal antibody (Product # PA5-85515) using 4 µg of sample at a dilution of 1:500. Sample was then incubated with anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATG9A Polyclonal Antibody immunoprecipitates ATG9A protein in IP experiments. IP samples: HeLa whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of ATG9A protein by 4 µg ATG9A Polyclonal Antibody (Product # PA5-85515). 7.5 % SDS-PAGE. The immunoprecipitated ATG9A protein was detected by ATG9A Polyclonal Antibody (Product # PA5-85515) diluted at 1:500.
