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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-85515 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG9A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Keep as concentrated solution. Predicted reactivity: Pig (100%), Rhesus Monkey (100%), Bovine (100%). Positive Control: 293T, A431, HeLa, RD. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.98 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATG9A Polyclonal Antibody detects ATG9A protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 100% MeOH for 5 min. Green: ATG9A protein stained by ATG9A Polyclonal Antibody (Product # PA5-85515) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of ATG9A in HeLa whole cell extract, A) control with preimmune rabbit IgG, B) immunoprecipitation of ATG9A protein with ATG9A polyclonal antibody (Product # PA5-85515) using 4 µg of sample at a dilution of 1:500. Sample was then incubated with anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATG9A Polyclonal Antibody immunoprecipitates ATG9A protein in IP experiments. IP samples: HeLa whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of ATG9A protein by 4 µg ATG9A Polyclonal Antibody (Product # PA5-85515). 7.5 % SDS-PAGE. The immunoprecipitated ATG9A protein was detected by ATG9A Polyclonal Antibody (Product # PA5-85515) diluted at 1:500.
