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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Other assay [1]
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- Product number
- PA1-25081 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Catenin alpha-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431 and cytosolic fraction of rat embryonic brain, MDCK, MCF-7. PA1-25081 is expected to cross react with mouse (100% conserved) and Xenopus laevis (100% conserved) due to sequence homology. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat, Canine, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Tight junctions negatively regulate mechanical forces applied to adherens junctions in vertebrate epithelial tissue.
Actomyosin-generated tension on cadherin is similar between dividing and non-dividing epithelial cells in early Xenopus laevis embryos.
Hatte G, Prigent C, Tassan JP
Journal of cell science 2018 Feb 5;131(3)
Journal of cell science 2018 Feb 5;131(3)
Actomyosin-generated tension on cadherin is similar between dividing and non-dividing epithelial cells in early Xenopus laevis embryos.
Herbomel G, Hatte G, Roul J, Padilla-Parra S, Tassan JP, Tramier M
Scientific reports 2017 Mar 22;7:45058
Scientific reports 2017 Mar 22;7:45058
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 EcadTSMod biosensor is integrated into the adherens junction and responds to several treatments. ( a ) Scatter plot of the fluorescence lifetime vs fluorescence intensity for each pixel of a representative acquisition. Equation of the linear regression is specified in the top right corner. ( b ) Indirect immunofluorescences of EcadTSMod detected with anti-GFP antibody (green) and alpha, beta and p120 catenins (red) in Xenopus laevis blastula overexpressing the EcadTSMod protein. Linescan were made on the indicated dotted line, and the normalized fluorescence intensity is shown for both channel (green for EcadTSMod and red for catenins) in the corresponding graph. Scale bar, 20 mum. ( c ) Boxplot of mean forces applied on EcadTSMod in untreated embryos or embryos treated with EGTA, latrunculin A, morpholino against alpha-catenin (MO alpha-catenin) and Calyculin A, with respectively 105, 32, 37, 44 and 29 independent fields (respective number of experiments: 14, 2, 2, 2 and 3; respective number of embryos: 55, 16, 19, 22 and 15). For each comparison, the p value is indicated. In the box plot, bold bars correspond to median value, whiskers to the value 1.5x away from the 1 st and 3 rd quartiles, and the dot to outlier.
