R31558

antibody from NSJ Bioreagents

Targeting: CTNNA1 CAP102

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31558

Provider

NSJ Bioreagents

Product name

Alpha Catenin Antibody

Antibody type

Polyclonal

Description

This highly specific Alpha Catenin antibody is suitable for use in Western blot/Immunohistochemistry/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the alpha Catenin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of alpha Catenin antibody and Lane 1: rat liver; 2: (r) lung; 3: (r) heart; 4: mouse NIH3T3; 5: (r) PC12; 6: human HEPG2; 7: (h) HeLa; 8: (h) MCF-7; 9: (m) HEPA. Expected/observed size ~102KD

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of alpha Catenin antibody and recombinant human protein (0.5ng)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of alpha Catenin antibody and Lane 1: rat liver; 2: (r) lung; 3: (r) heart; 4: mouse NIH3T3; 5: (r) PC12; 6: human HEPG2; 7: (h) HeLa; 8: (h) MCF-7; 9: (m) HEPA. Expected/observed size ~102KD

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of alpha Catenin antibody and recombinant human protein (0.5ng)

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with alpha Catenin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: alpha Catenin antibody testing of human breast tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: alpha Catenin antibody testing of mouse intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P staining of rat intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: alpha Catenin antibody testing of human breast tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: alpha Catenin antibody testing of mouse intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P staining of rat intestine tissue

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human U-87 MG cells with alpha Catenin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= alpha Catenin antibody.