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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ5638 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Alpha 1 Catenin Antibody / CTNNA1
- Antibody type
- Monoclonal
- Description
- This highly specific Alpha 1 Catenin antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 10I2
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Alpha 1 Catenin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) HEK293, 3) U-2 OS, 4) U-87 MG, 5) rat PC-12 and 6) mouse NIH 3T3 lysate with Alpha 1 Catenin antibody. Predicted molecular weight ~102 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast cancer with Alpha 1 Catenin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human Jurkat cells with Alpha 1 Catenin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Alpha 1 Catenin antibody.
