PA5-90334

antibody from Invitrogen Antibodies

Targeting: CTNNA1 CAP102

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-90334

Provider

Invitrogen Antibodies

Product name

Catenin alpha-1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: TNEQDLANRF KEFGKEMVKL NYVAARRQQE LKDPHCRDEM AAARGALKKN ATMLYTASQA FLRHPDVAAT RANRDYVFKQ VQEAIAGISN AAQATSPTDE AKGHTGIGEL AAALNEFDNK IILD Positive Samples: Mouse brain, Rat brain; Cellular Location: Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, axon, cytoskeleton

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.22 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Catenin alpha-1 in extracts of various cell lines. Samples were incubated with Catenin alpha-1 Polyclonal antibody (Product # PA5-90334) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Catenin alpha-1 in Neuro-2a cells. Samples were incubated with Catenin alpha-1 Polyclonal antibody (Product # PA5-90334) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Catenin alpha-1 in PC-12 cells. Samples were incubated with Catenin alpha-1 Polyclonal antibody (Product # PA5-90334) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Catenin alpha-1 in paraffin-embedded rat liver. Samples were incubated with Catenin alpha-1 Polyclonal antibody (Product # PA5-90334) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Catenin alpha-1 in paraffin-embedded mouse spinal cord. Samples were incubated with Catenin alpha-1 Polyclonal antibody (Product # PA5-90334) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Catenin alpha-1 in paraffin-embedded mouse liver. Samples were incubated with Catenin alpha-1 Polyclonal antibody (Product # PA5-90334) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.