Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- AX10007 - Provider product page
- Provider
- Abcepta
- Product name
- CTNNB1 Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "CTNNB1 Antibody (C-term) (Cat. #AX10007) western blot analysis in 293,T47D cell line lysates (35ug/lane).This demonstrates the CTNNB1 antibody detected the CTNNB1 protein (arrow)."
- Primary Ab dilution
- 1:2000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of CTNNB1 Antibody (C-term) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- CTNNB1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CTNNB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- CTNNB1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CTNNB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- CTNNB1 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:50