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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- MAB13292 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal beta-Catenin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat beta-Catenin in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat beta-Catenin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse/Rat beta-Catenin Monoclonal Antibody (Catalog # MAB13292) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for beta-Catenin at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of beta-Catenin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Human/Mouse/Rat beta-Catenin Monoclonal Antibody (Catalog # MAB13292, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
