Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MA1-34999 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta Catenin Monoclonal Antibody (15B8), Cyanine3
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 15B8
- Vial size
- 100 µL
- Concentration
- 1.1 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-beta Catenin Monoclonal Antibody (15B8), Cyanine3 (Product # MA1-34999) and a 85 kDa band corresponding to Catenin beta-1 was observed across the cell lines tested and decreased upon TGF-ß1 for 24 hours in MCF7 cells along with a non specific band at 60 kDa. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), SK-MEL-31 (Lane 2), MCF7 (Lane 3), MCF7 treated with 10 ng/mL of TGF-ß1 for 24 hours (Lane 4), A-431 (Lane 5) and HeLa (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody at 1:1000 dilution and detected by fluorescence with absorption wavelength at 552 nm emission wavelength at 565 nm using the iBright FL 1000 (Product # A32752).