MA1-34999

antibody from Invitrogen Antibodies

Targeting: CTNNB1 armadillo, beta-catenin, CTNNB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-34999

Provider

Invitrogen Antibodies

Product name

beta Catenin Monoclonal Antibody (15B8), Cyanine3

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian

Host

Mouse

Isotype

IgG

Antibody clone number

15B8

Vial size

100 µL

Concentration

1.1 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-beta Catenin Monoclonal Antibody (15B8), Cyanine3 (Product # MA1-34999) and a 85 kDa band corresponding to Catenin beta-1 was observed across the cell lines tested and decreased upon TGF-ß1 for 24 hours in MCF7 cells along with a non specific band at 60 kDa. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), SK-MEL-31 (Lane 2), MCF7 (Lane 3), MCF7 treated with 10 ng/mL of TGF-ß1 for 24 hours (Lane 4), A-431 (Lane 5) and HeLa (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody at 1:1000 dilution and detected by fluorescence with absorption wavelength at 552 nm emission wavelength at 565 nm using the iBright FL 1000 (Product # A32752).