Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-36827 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-beta Catenin (Ser37) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 92 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Identification of KIF4A as a pan-cancer diagnostic and prognostic biomarker via bioinformatics analysis and validation in osteosarcoma cell lines.
Pan J, Lei X, Mao X
PeerJ 2021;9:e11455
PeerJ 2021;9:e11455
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-beta-Catenin pSer37 using Phospho-beta-Catenin pSer37 polyclonal antibody (Product # PA5-36827) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with PMA, Lane 2: H9C2 cell lysate treated with PMA.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-beta Catenin (Ser37) in Lane 1: HEK293T cell lysate treated with PMA, Lane 2: H9C2 cell lysate treated with PMA. Samples were incubated with Phospho-beta Catenin (Ser37) polyclonal antibody (Product # PA5-36827) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-beta-Catenin pSer37 in paraffin-embedded human breast carcinoma using Phospho-beta-Catenin pSer37 polyclonal antibody (Product # PA5-36827).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 10.7717/peerj.11455/fig-5 Figure 5 Silencing KIF4A induced apoptosis and cell cycle arrest of Osteosarcoma cells. (A-L) Annexin V-FITC and PI was used to evaluate apoptosis rate. (M-X) Cell cycle was detected by flow cytometry. (Y-BB)The expression of apoptosis-related proteins Bax and Bcl-2 and caspase-3 were determined by Western blot analysis. * P < 0.05 compared with Control, # P < 0.05 compared with si-NC, & P < 0.05 compared with oe-NC.