Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-37543 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-beta Catenin (Ser33) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is MCF-7 cells; suggested positive control for IHC is human breast carcinoma.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Tissue-specific transplantation antigen P35B functions as an oncogene and is regulated by microRNA-125a-5p in lung cancer.
Gao Y, Zhang G, Liu J, Li H
Oncology reports 2021 May;45(5)
Oncology reports 2021 May;45(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from MCF-7 cells, untreated or treated with Calyculin A, using beta-Catenin (pSer33) polyclonal antibody (Product # PA5-37543).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-beta Catenin (Ser33) in paraffin-embedded Human breast carcinoma tissue using Phospho-beta Catenin (Ser33) Polyclonal Antibody (Product # PA5-37543) (left) or the same antibody preincubated with blocking peptide (right).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. TSTA3 overexpression increases beta-catenin expression and nuclear accumulation in NCI-H1299 cells. NCI-H1299 cells were transfected with OE-NC or OE-TSTA3, and then western blotting and RT-qPCR were used to detect the expression levels of (A) proteins and (B) mRNAs. (C) The subcellular location of beta-catenin protein was assessed via immunofluorescence assay. Scale bar, 5 um. (D) The expression levels of beta-catenin and p-beta-catenin were determined via western blotting. NCI-H1299 cells were transfected with sh-NC or sh-TSTA3, and then western blotting and RT-qPCR were used to detect the expression levels of (E) proteins and (F) mRNAs. (G) The subcellular location of beta-catenin protein was assessed via immunofluorescence assay. Scale bar, 5 um. (H) The expression levels of beta-catenin and p-beta-catenin were determined via western blotting. *P