PA5-37543

antibody from Invitrogen Antibodies

Targeting: CTNNB1 armadillo, beta-catenin, CTNNB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-37543

Provider

Invitrogen Antibodies

Product name

Phospho-beta Catenin (Ser33) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control for Western blot is MCF-7 cells; suggested positive control for IHC is human breast carcinoma.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Tissue-specific transplantation antigen P35B functions as an oncogene and is regulated by microRNA-125a-5p in lung cancer.

Gao Y, Zhang G, Liu J, Li H
Oncology reports 2021 May;45(5)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts from MCF-7 cells, untreated or treated with Calyculin A, using beta-Catenin (pSer33) polyclonal antibody (Product # PA5-37543).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-beta Catenin (Ser33) in paraffin-embedded Human breast carcinoma tissue using Phospho-beta Catenin (Ser33) Polyclonal Antibody (Product # PA5-37543) (left) or the same antibody preincubated with blocking peptide (right).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4. TSTA3 overexpression increases beta-catenin expression and nuclear accumulation in NCI-H1299 cells. NCI-H1299 cells were transfected with OE-NC or OE-TSTA3, and then western blotting and RT-qPCR were used to detect the expression levels of (A) proteins and (B) mRNAs. (C) The subcellular location of beta-catenin protein was assessed via immunofluorescence assay. Scale bar, 5 um. (D) The expression levels of beta-catenin and p-beta-catenin were determined via western blotting. NCI-H1299 cells were transfected with sh-NC or sh-TSTA3, and then western blotting and RT-qPCR were used to detect the expression levels of (E) proteins and (F) mRNAs. (G) The subcellular location of beta-catenin protein was assessed via immunofluorescence assay. Scale bar, 5 um. (H) The expression levels of beta-catenin and p-beta-catenin were determined via western blotting. *P