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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- UM500014 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500014, RRID:AB_2629029
- Product name
- Beta-Catenin (CTNNB1) mouse monoclonal antibody, clone UMAB14
- Antibody type
- Monoclonal
- Description
- Beta-Catenin (CTNNB1) mouse monoclonal antibody, clone UMAB14
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CTNNB1
- Isotype
- IgG
- Antibody clone number
- UMAB14
- Vial size
- 100 µl
- Concentration
- 0.53 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western analysis of HEK 293T cells without treatment or treated with 30mM LiCl for 16 hours, using anti-beta-catenin antibody (clone UMAB14). Anti-HSP90 (TA500494) was used as internal loading control.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody (Clone UMAB14).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of 10 different human tissue lysates (10ug) by using Anti-?-Catenin monoclonal antibody (Clone UMAB14, 1:500)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- MDCK cells were treated either with 30mM NaCl as negative control (left) or with GSK-3beta kinase inhibor 30mM LiCl (right) for 48 hrs. Anti-beta-catenin monoclonal antibody (clone UMAB14) was used for immunofluorescent staining.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human normal lung and lung Adenocarcinoma tissues using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB14, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3mi
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human normal ovary and Adenocarcinoma of the ovary tissues using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB14, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Mouse colon tissue using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB14, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-CTNNB1 antibody(UM500014),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-beta-catenin mouse monoclonal antibody (Clone UMAB14). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-beta-catenin (Clone UMAB14) very specifically recognizes beta-catenin antigen on OriGene protein microarray chip.
- Validation comment
- 10K-CHIP
