Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 12-2567-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta Catenin Monoclonal Antibody (15B8), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway. In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt and beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells.
- Conjugate
- Yellow dye
- Antibody clone number
- 15B8
- Concentration
- 5 µL/Test
Submitted references Wnt activation promotes memory T cell polyfunctionality via epigenetic regulator PRMT1.
Sung BY, Lin YH, Kong Q, Shah PD, Glick Bieler J, Palmer S, Weinhold KJ, Chang HR, Huang H, Avery RK, Schneck J, Chiu YL
The Journal of clinical investigation 2022 Jan 18;132(2)
The Journal of clinical investigation 2022 Jan 18;132(2)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of Jurkat cell line with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human/Mouse beta-Catenin PE (purple histogram). Total viable cells were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Wnt signaling alters human memory CD8 + T cell proliferation and differentiation. ( A ) FACS-sorted human naive and memory CD8 + T cells were stained with CTV and stimulated with CD3/CD28 in DMSO (black), TWS119 (blue), and SKL2001 (red) for 7 days. Numbers represent the percentages of divided cells in each treatment. ( B ) Average proliferative response for each treatment ( n = 5). Dunn's test for multiple comparisons. ( C ) Memory CD8 + T cells in different treatments for 7 days were stained with phenotypic markers. Numbers represent the percentages of cells in each quadrant. ( D ) Quantitative RT-PCR analysis of Tcf7, Lef1, and Fzd7 in memory CD8 + T cells with or without Wnt agonist treatment on days 1, 3, and 5. Numbers are normalized to marker expression of the DMSO group at each corresponding time point. ( E ) Memory CD8 + T cells were stimulated with CD3/CD28 and treated with or without Wnt agonists for 7 days. beta-Catenin and TCF1 levels were analyzed by flow cytometry. Numbers represent the MFI of each protein. ( F ) Average MFI of beta-catenin and TCF1 in different treatment conditions ( n = 4). Dunn's test for multiple comparisons. # P < 0.05.
- Conjugate
- Yellow dye