14-2567-80

antibody from Invitrogen Antibodies
Targeting: CTNNB1 armadillo, beta-catenin, CTNNB
Provider product page for 14-2567-80
Western blot
Immunoprecipitation
Immunohistochemistry
Flow cytometry

Antibody data

Product number
14-2567-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-beta Catenin Monoclonal Antibody (15B8), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway. In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt and beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells. Applications Reported: This 15B8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated 15B8 is recommended for use in intracellular flow cytometry.). Applications Tested: This 15B8 antibody has been tested by western blot analysis on reduced cell lysates prepared from the Jurkat cell line. This antibody can be used at 1-5 µg/mL. This antibody has been tested by immunohistchemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse
Host
Mouse
Isotype
IgG
Antibody clone number
15B8
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C
CTNNB1 protein structure - 14-2567-80 shown in red.