Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-67504 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-beta Catenin (Ser715) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with Mouse and Rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.
Malgundkar SH, Burney I, Al Moundhri M, Al Kalbani M, Lakhtakia R, Okamoto A, Tamimi Y
BMC cancer 2020 May 4;20(1):374
BMC cancer 2020 May 4;20(1):374
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-beta Catenin (Ser715) in extracts from U87 cells transfected with either PKCd siRNA targeting or control siRNA treated with Wnt3a (100 ng mL 1) for 8 h. WB was performed with nuclearlysates of the cells using a Phospho-beta Catenin (Ser715) Polyclonal Antibody (Product # PA5-67504).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-beta Catenin (Ser715) in extracts from U87 cells transfected with either PKCd siRNA targeting or control siRNA treated with Wnt3a (100 ng mL 1) for 8 h. WB was performed with nuclearlysates of the cells using a Phospho-beta Catenin (Ser715) Polyclonal Antibody (Product # PA5-67504).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-beta Catenin (Ser715) in paraffin-embedded human primary glioblastoma multiforme (GBM) specimens using Phospho-beta Catenin (Ser715) Polyclonal Antibody (Product # PA5-67504).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Regulatory effects of FAT4 on the expression of proteins involved in EMT, Hippo, Wnt-beta-catenin, apoptotic, and retinoblastoma pathways by Western blotting. a . Relative expression variation of proteins. The expression of Vimentin ( p = 0.0001), YAP ( p = 0.0018), beta-catenin ( p = 0.001), Bcl2 ( p = 0.0001), cyclin D1 ( p = 0.0017) and cdk4 ( p = 0.0025) was higher in FAT4 siRNA treated cells as compared to control. beta-actin was used as an internal control. b . Western blot demonstrating bands for protein expression in control and FAT4 knocked cells. Full-length blots are presented in Supplementary figure S 1 . c . The ratio of phosphorylated to total YAP, GSK-3beta, beta-catenin, and retinoblastoma proteins following FAT4 repression. The pYAP/ YAP ratio was lower in FAT4 siRNA treated cells as compared to the control ( p = 0.0286). Similarly, pGSK-3-beta/GSK-3-beta ratio, and pbeta-catenin/beta-catenin ratio was lower in FAT4 siRNA treated cells ( p = 0.018, and p = 0.001 respectively) as compared to control. There was no significant difference in pRb/Rb ratio between FAT4 siRNA treated cells and control. MCAS cells treated with scrambled siRNA was used as control. Data represent mean and standard deviation from at least three independent experiments performed in triplicates