V3601

antibody from NSJ Bioreagents

Targeting: CTNNB1 armadillo, beta-catenin, CTNNB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V3601

Provider

NSJ Bioreagents

Product name

Recombinant Beta Catenin Antibody

Antibody type

Monoclonal

Description

This highly specific Recombinant Beta Catenin antibody is suitable for use in Immunofluorescence/Western blot/Immunohistochemistry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

rCTNNB1/2173

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Store the recombinant beta Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HeLa cell lysate with recombinant beta Catenin antibody. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human brain tissue lysate with recombinant beta Catenin antibody. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of human HeLa cells with recombinant beta Catenin antibody (clone rCTNNB1/2173, green) and Reddot nuclear stain (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast cancer with recombinant beta Catenin antibody (clone rCTNNB1/2173). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant beta Catenin antibody (clone rCTNNB1/2173). These results demonstrate the foremost specificity of the rCTNNB1/2173 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free recombinant beta Catenin antibody (clone rCTNNB1/2173) as confirmation of integrity and purity.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free recombinant beta Catenin antibody (clone rCTNNB1/2173) as confirmation of integrity and purity.