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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [2]
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- Product number
- H00001499-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001499-M02, RRID:AB_920810
- Product name
- Mouse Monoclonal beta-Catenin Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CTNNB1 (1C9)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of CTNNB1 over-expressed 293 cell line, cotransfected with CTNNB1 Validated Chimera RNAi ( Cat # H00001499-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CTNNB1 monoclonal antibody (M02), clone 1C9 (Cat # H00001499-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of CTNNB1 expression in transfected 293T cell line by CTNNB1 monoclonal antibody (M02), clone 1C9.Lane 1: CTNNB1 transfected lysate(85.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: beta-Catenin Antibody (1C9) [H00001499-M02] - Detection limit for recombinant GST tagged CTNNB1 is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (1C9) [H00001499-M02] - PC-3 MM2 cells were stained with CTNNB1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of monoclonal antibody to CTNNB1 on HeLa cell. Antibody concentration 10 ug/ml.
