M00004-2

antibody from Boster Biological Technology

Targeting: CTNNB1 armadillo, beta-catenin, CTNNB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00004-2

Provider

Boster Biological Technology

Product name

Anti-beta Catenin CTNNB1 Antibody Picoband™ (monoclonal, 1F6)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for beta Catenin detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

1F6

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

VEGF overexpression in transplanted NSCs promote recovery of neurological function in rats with cerebral ischemia by modulating the Wnt signal transduction pathway.

Zhu Y, Liu R, Zhao X, Kang C, Yang D, Ge G
Neuroscience letters 2024 Feb 28;824:137668

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SIRT6 Promotes the Progression of Prostate Cancer via Regulating the Wnt/β-Catenin Signaling Pathway.

Zhang X, Chen R, Song LD, Zhu LF, Zhan JF
Journal of oncology 2022;2022:2174758

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Nuciferine Inhibits TMEM16A in Dietary Adjuvant Therapy for Lung Cancer.

Bai X, Liu X, Li S, An H, Kang X, Guo S
Journal of agricultural and food chemistry 2022 Mar 30;70(12):3687-3696

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Sdy-1 Executes Antitumor Activity in HepG2 and HeLa Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway.

Sun M, Zhou D, Wu J, Zhou J, Xu J
Marine drugs 2022 Feb 5;20(2)

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Expression and clinical significance of HOXA5, E-cadherin, and β-catenin in cervical squamous cell carcinoma.

Pei L, Wang ZQ, Shen J, Yang YZ, Tian J, He X, Lin J, Hou QY, Mo WF, Zhao HL, Zhou YQ
International journal of clinical and experimental pathology 2018;11(6):3091-3096

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Up-regulation of E-cadherin by saRNA inhibits the migration and invasion of renal carcinoma cells.

Dai J, He H, Lin D, Wang C, Zhu Y, Xu D
International journal of clinical and experimental pathology 2018;11(12):5792-5800

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Effect of FH535 on in vitro maturation of porcine oocytes by inhibiting WNT signaling pathway.

Shi M, Cheng J, He Y, Jiang Z, Bodinga BM, Liu B, Chen H, Li Q
Animal science journal = Nihon chikusan Gakkaiho 2018 Apr;89(4):631-639

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Stimulating effect of a newly synthesized sulfonamido-based gallate on articular chondrocytes in vitro.

Lu Z, Wang L, Pan H, Lin X, Lin C, Liu B, Zheng L, Zhao J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2015;37(3):1196-209

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LiCl inhibits PRRSV infection by enhancing Wnt/β-catenin pathway and suppressing inflammatory responses.

Hao HP, Wen LB, Li JR, Wang Y, Ni B, Wang R, Wang X, Sun MX, Fan HJ, Mao X
Antiviral research 2015 May;117:99-109

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Lithium chloride decreases proliferation and migration of C6 glioma cells harboring isocitrate dehydrogenase 2 mutant via GSK-3β.

Fu Y, Zheng Y, Chan KG, Liang A, Hu F
Molecular biology reports 2014 Jun;41(6):3907-13

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human 22RV1 whole cell lysates, Lane 7: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-beta Catenin antigen affinity purified monoclonal antibody (Catalog # M00004-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.

Additional image