Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [39]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 05-665 - Provider product page
- Provider
- EMD Millipore
- Proper citation
- Millipore Cat#05-665, RRID:AB_309887
- Product name
- Anti-Active-?-Catenin (Anti-ABC) Antibody, clone 8E7
- Antibody type
- Monoclonal
- Antigen
- Fusion protein corresponding to residues 1-100 of human ?-catenin with a c-terminal histidine tag
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8,00E+07
- Vial size
- 100 µg
- Storage
- Maintain for 1 year at 2-8°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Submitted references Wnt/β-catenin signalling regulates Sox17 expression and is essential for organizer and endoderm formation in the mouse.
COUP-TFII controls mouse pancreatic β-cell mass through GLP-1-β-catenin signaling pathways.
Cadmium induces carcinogenesis in BEAS-2B cells through ROS-dependent activation of PI3K/AKT/GSK-3β/β-catenin signaling.
Inhibition of cardiomyocytes differentiation of mouse embryonic stem cells by CD38/cADPR/Ca2+ signaling pathway.
E-cadherin is required for intestinal morphogenesis in the mouse.
Hematopoietic stem cell development requires transient Wnt/β-catenin activity.
Wnt activation downregulates olfactomedin-1 in Fallopian tubal epithelial cells: a microenvironment predisposed to tubal ectopic pregnancy.
Inhibition of Wnt/β-catenin signaling by dexamethasone promotes adipocyte differentiation in mesenchymal progenitor cells, ROB-C26.
The human cadherin 11 is a pro-apoptotic tumor suppressor modulating cell stemness through Wnt/β-catenin signaling and silenced in common carcinomas.
Splicing of histone deacetylase 7 modulates smooth muscle cell proliferation and neointima formation through nuclear β-catenin translocation.
Wnt10b activates the Wnt, notch, and NFκB pathways in U2OS osteosarcoma cells.
Hepatitis C virus core protein activates Wnt/β-catenin signaling through multiple regulation of upstream molecules in the SMMC-7721 cell line.
Wnt/β-catenin pathway in podocytes integrates cell adhesion, differentiation, and survival.
The chromatin-remodeling enzyme BRG1 modulates vascular Wnt signaling at two levels.
Canonical Wnt/β-catenin signaling mediates transforming growth factor-β1-driven podocyte injury and proteinuria.
Parathyroid hormone receptor directly interacts with dishevelled to regulate beta-Catenin signaling and osteoclastogenesis.
Loss of hepatocyte-nuclear-factor-1alpha impacts on adult mouse intestinal epithelial cell growth and cell lineages differentiation.
Differential activation of canonical Wnt signaling determines cranial sutures fate: a novel mechanism for sagittal suture craniosynostosis.
A p120-catenin-CK1epsilon complex regulates Wnt signaling.
Histone deacetylase 7 controls endothelial cell growth through modulation of beta-catenin.
Slug gene expression supports human osteoblast maturation.
Epigenetic alteration of Wnt pathway antagonists in progressive glandular neoplasia of the lung.
Dickkopf-1 secreted by decidual cells promotes trophoblast cell invasion during murine placentation.
Wnt4 inhibits beta-catenin/TCF signalling by redirecting beta-catenin to the cell membrane.
Effects of Wnt proteins on cell proliferation and apoptosis in HEK293 cells.
A rational mechanism for combination treatment of Huntington's disease using lithium and rapamycin.
The SIRT1 deacetylase suppresses intestinal tumorigenesis and colon cancer growth.
Effects of Wnt3a on proliferation and differentiation of human epidermal stem cells.
Leukocytes induce epithelial to mesenchymal transition after unilateral ureteral obstruction in neonatal mice.
Wnt signaling and phosphorylation status of beta-catenin: importance of the correct antibody tools.
The activation of beta-catenin by Wnt signaling mediates the effects of histone deacetylase inhibitors.
GFP transgenic mice reveal active canonical Wnt signal in neonatal brain and in adult liver and spleen.
Transcriptional profiling of mammary gland side population cells.
Enrichment and characterization of mouse putative epidermal stem cells.
Canonical Wnt signaling is critical to estrogen-mediated uterine growth.
Enrichment and identification of human 'fetal' epidermal stem cells.
Alterations of beta-catenin pathway in non-melanoma skin tumors: loss of alpha-ABC nuclear reactivity correlates with the presence of beta-catenin gene mutation.
Wnt signaling controls the phosphorylation status of beta-catenin.
Wnt signals are transmitted through N-terminally dephosphorylated beta-catenin.
Engert S, Burtscher I, Liao WP, Dulev S, Schotta G, Lickert H
Development (Cambridge, England) 2013 Aug;140(15):3128-38
Development (Cambridge, England) 2013 Aug;140(15):3128-38
COUP-TFII controls mouse pancreatic β-cell mass through GLP-1-β-catenin signaling pathways.
Boutant M, Ramos OH, Tourrel-Cuzin C, Movassat J, Ilias A, Vallois D, Planchais J, Pégorier JP, Schuit F, Petit PX, Bossard P, Maedler K, Grapin-Botton A, Vasseur-Cognet M
PloS one 2012;7(1):e30847
PloS one 2012;7(1):e30847
Cadmium induces carcinogenesis in BEAS-2B cells through ROS-dependent activation of PI3K/AKT/GSK-3β/β-catenin signaling.
Son YO, Wang L, Poyil P, Budhraja A, Hitron JA, Zhang Z, Lee JC, Shi X
Toxicology and applied pharmacology 2012 Oct 15;264(2):153-60
Toxicology and applied pharmacology 2012 Oct 15;264(2):153-60
Inhibition of cardiomyocytes differentiation of mouse embryonic stem cells by CD38/cADPR/Ca2+ signaling pathway.
Wei WJ, Sun HY, Ting KY, Zhang LH, Lee HC, Li GR, Yue J
The Journal of biological chemistry 2012 Oct 12;287(42):35599-611
The Journal of biological chemistry 2012 Oct 12;287(42):35599-611
E-cadherin is required for intestinal morphogenesis in the mouse.
Bondow BJ, Faber ML, Wojta KJ, Walker EM, Battle MA
Developmental biology 2012 Nov 1;371(1):1-12
Developmental biology 2012 Nov 1;371(1):1-12
Hematopoietic stem cell development requires transient Wnt/β-catenin activity.
Ruiz-Herguido C, Guiu J, D'Altri T, Inglés-Esteve J, Dzierzak E, Espinosa L, Bigas A
The Journal of experimental medicine 2012 Jul 30;209(8):1457-68
The Journal of experimental medicine 2012 Jul 30;209(8):1457-68
Wnt activation downregulates olfactomedin-1 in Fallopian tubal epithelial cells: a microenvironment predisposed to tubal ectopic pregnancy.
Kodithuwakku SP, Pang RT, Ng EH, Cheung AN, Horne AW, Ho PC, Yeung WS, Lee KF
Laboratory investigation; a journal of technical methods and pathology 2012 Feb;92(2):256-64
Laboratory investigation; a journal of technical methods and pathology 2012 Feb;92(2):256-64
Inhibition of Wnt/β-catenin signaling by dexamethasone promotes adipocyte differentiation in mesenchymal progenitor cells, ROB-C26.
Naito M, Omoteyama K, Mikami Y, Takahashi T, Takagi M
Histochemistry and cell biology 2012 Dec;138(6):833-45
Histochemistry and cell biology 2012 Dec;138(6):833-45
The human cadherin 11 is a pro-apoptotic tumor suppressor modulating cell stemness through Wnt/β-catenin signaling and silenced in common carcinomas.
Li L, Ying J, Li H, Zhang Y, Shu X, Fan Y, Tan J, Cao Y, Tsao SW, Srivastava G, Chan AT, Tao Q
Oncogene 2012 Aug 23;31(34):3901-12
Oncogene 2012 Aug 23;31(34):3901-12
Splicing of histone deacetylase 7 modulates smooth muscle cell proliferation and neointima formation through nuclear β-catenin translocation.
Zhou B, Margariti A, Zeng L, Habi O, Xiao Q, Martin D, Wang G, Hu Y, Wang X, Xu Q
Arteriosclerosis, thrombosis, and vascular biology 2011 Nov;31(11):2676-84
Arteriosclerosis, thrombosis, and vascular biology 2011 Nov;31(11):2676-84
Wnt10b activates the Wnt, notch, and NFκB pathways in U2OS osteosarcoma cells.
Mödder UI, Oursler MJ, Khosla S, Monroe DG
Journal of cellular biochemistry 2011 May;112(5):1392-402
Journal of cellular biochemistry 2011 May;112(5):1392-402
Hepatitis C virus core protein activates Wnt/β-catenin signaling through multiple regulation of upstream molecules in the SMMC-7721 cell line.
Liu J, Wang Z, Tang J, Tang R, Shan X, Zhang W, Chen Q, Zhou F, Chen K, Huang A, Tang N
Archives of virology 2011 Jun;156(6):1013-23
Archives of virology 2011 Jun;156(6):1013-23
Wnt/β-catenin pathway in podocytes integrates cell adhesion, differentiation, and survival.
Kato H, Gruenwald A, Suh JH, Miner JH, Barisoni-Thomas L, Taketo MM, Faul C, Millar SE, Holzman LB, Susztak K
The Journal of biological chemistry 2011 Jul 22;286(29):26003-15
The Journal of biological chemistry 2011 Jul 22;286(29):26003-15
The chromatin-remodeling enzyme BRG1 modulates vascular Wnt signaling at two levels.
Griffin CT, Curtis CD, Davis RB, Muthukumar V, Magnuson T
Proceedings of the National Academy of Sciences of the United States of America 2011 Feb 8;108(6):2282-7
Proceedings of the National Academy of Sciences of the United States of America 2011 Feb 8;108(6):2282-7
Canonical Wnt/β-catenin signaling mediates transforming growth factor-β1-driven podocyte injury and proteinuria.
Wang D, Dai C, Li Y, Liu Y
Kidney international 2011 Dec;80(11):1159-69
Kidney international 2011 Dec;80(11):1159-69
Parathyroid hormone receptor directly interacts with dishevelled to regulate beta-Catenin signaling and osteoclastogenesis.
Romero G, Sneddon WB, Yang Y, Wheeler D, Blair HC, Friedman PA
The Journal of biological chemistry 2010 May 7;285(19):14756-63
The Journal of biological chemistry 2010 May 7;285(19):14756-63
Loss of hepatocyte-nuclear-factor-1alpha impacts on adult mouse intestinal epithelial cell growth and cell lineages differentiation.
Lussier CR, Brial F, Roy SA, Langlois MJ, Verdu EF, Rivard N, Perreault N, Boudreau F
PloS one 2010 Aug 24;5(8):e12378
PloS one 2010 Aug 24;5(8):e12378
Differential activation of canonical Wnt signaling determines cranial sutures fate: a novel mechanism for sagittal suture craniosynostosis.
Behr B, Longaker MT, Quarto N
Developmental biology 2010 Aug 15;344(2):922-40
Developmental biology 2010 Aug 15;344(2):922-40
A p120-catenin-CK1epsilon complex regulates Wnt signaling.
Casagolda D, Del Valle-Pérez B, Valls G, Lugilde E, Vinyoles M, Casado-Vela J, Solanas G, Batlle E, Reynolds AB, Casal JI, de Herreros AG, Duñach M
Journal of cell science 2010 Aug 1;123(Pt 15):2621-31
Journal of cell science 2010 Aug 1;123(Pt 15):2621-31
Histone deacetylase 7 controls endothelial cell growth through modulation of beta-catenin.
Margariti A, Zampetaki A, Xiao Q, Zhou B, Karamariti E, Martin D, Yin X, Mayr M, Li H, Zhang Z, De Falco E, Hu Y, Cockerill G, Xu Q, Zeng L
Circulation research 2010 Apr 16;106(7):1202-11
Circulation research 2010 Apr 16;106(7):1202-11
Slug gene expression supports human osteoblast maturation.
Lambertini E, Lisignoli G, Torreggiani E, Manferdini C, Gabusi E, Franceschetti T, Penolazzi L, Gambari R, Facchini A, Piva R
Cellular and molecular life sciences : CMLS 2009 Nov;66(22):3641-53
Cellular and molecular life sciences : CMLS 2009 Nov;66(22):3641-53
Epigenetic alteration of Wnt pathway antagonists in progressive glandular neoplasia of the lung.
Licchesi JD, Westra WH, Hooker CM, Machida EO, Baylin SB, Herman JG
Carcinogenesis 2008 May;29(5):895-904
Carcinogenesis 2008 May;29(5):895-904
Dickkopf-1 secreted by decidual cells promotes trophoblast cell invasion during murine placentation.
Peng S, Li J, Miao C, Jia L, Hu Z, Zhao P, Li J, Zhang Y, Chen Q, Duan E
Reproduction (Cambridge, England) 2008 Mar;135(3):367-75
Reproduction (Cambridge, England) 2008 Mar;135(3):367-75
Wnt4 inhibits beta-catenin/TCF signalling by redirecting beta-catenin to the cell membrane.
Bernard P, Fleming A, Lacombe A, Harley VR, Vilain E
Biology of the cell / under the auspices of the European Cell Biology Organization 2008 Mar;100(3):167-77
Biology of the cell / under the auspices of the European Cell Biology Organization 2008 Mar;100(3):167-77
Effects of Wnt proteins on cell proliferation and apoptosis in HEK293 cells.
Jia L, Miao C, Cao Y, Duan EK
Cell biology international 2008 Jul;32(7):807-13
Cell biology international 2008 Jul;32(7):807-13
A rational mechanism for combination treatment of Huntington's disease using lithium and rapamycin.
Sarkar S, Krishna G, Imarisio S, Saiki S, O'Kane CJ, Rubinsztein DC
Human molecular genetics 2008 Jan 15;17(2):170-8
Human molecular genetics 2008 Jan 15;17(2):170-8
The SIRT1 deacetylase suppresses intestinal tumorigenesis and colon cancer growth.
Firestein R, Blander G, Michan S, Oberdoerffer P, Ogino S, Campbell J, Bhimavarapu A, Luikenhuis S, de Cabo R, Fuchs C, Hahn WC, Guarente LP, Sinclair DA
PloS one 2008 Apr 16;3(4):e2020
PloS one 2008 Apr 16;3(4):e2020
Effects of Wnt3a on proliferation and differentiation of human epidermal stem cells.
Jia L, Zhou J, Peng S, Li J, Cao Y, Duan E
Biochemical and biophysical research communications 2008 Apr 11;368(3):483-8
Biochemical and biophysical research communications 2008 Apr 11;368(3):483-8
Leukocytes induce epithelial to mesenchymal transition after unilateral ureteral obstruction in neonatal mice.
Lange-Sperandio B, Trautmann A, Eickelberg O, Jayachandran A, Oberle S, Schmidutz F, Rodenbeck B, Hömme M, Horuk R, Schaefer F
The American journal of pathology 2007 Sep;171(3):861-71
The American journal of pathology 2007 Sep;171(3):861-71
Wnt signaling and phosphorylation status of beta-catenin: importance of the correct antibody tools.
van Noort M, Weerkamp F, Clevers HC, Staal FJ
Blood 2007 Oct 1;110(7):2778-9
Blood 2007 Oct 1;110(7):2778-9
The activation of beta-catenin by Wnt signaling mediates the effects of histone deacetylase inhibitors.
Bordonaro M, Lazarova DL, Sartorelli AC
Experimental cell research 2007 May 1;313(8):1652-66
Experimental cell research 2007 May 1;313(8):1652-66
GFP transgenic mice reveal active canonical Wnt signal in neonatal brain and in adult liver and spleen.
Moriyama A, Kii I, Sunabori T, Kurihara S, Takayama I, Shimazaki M, Tanabe H, Oginuma M, Fukayama M, Matsuzaki Y, Saga Y, Kudo A
Genesis (New York, N.Y. : 2000) 2007 Feb;45(2):90-100
Genesis (New York, N.Y. : 2000) 2007 Feb;45(2):90-100
Transcriptional profiling of mammary gland side population cells.
Behbod F, Xian W, Shaw CA, Hilsenbeck SG, Tsimelzon A, Rosen JM
Stem cells (Dayton, Ohio) 2006 Apr;24(4):1065-74
Stem cells (Dayton, Ohio) 2006 Apr;24(4):1065-74
Enrichment and characterization of mouse putative epidermal stem cells.
Zhou JX, Jia LW, Yang YJ, Peng S, Cao YJ, Duan EK
Cell biology international 2004;28(7):523-9
Cell biology international 2004;28(7):523-9
Canonical Wnt signaling is critical to estrogen-mediated uterine growth.
Hou X, Tan Y, Li M, Dey SK, Das SK
Molecular endocrinology (Baltimore, Md.) 2004 Dec;18(12):3035-49
Molecular endocrinology (Baltimore, Md.) 2004 Dec;18(12):3035-49
Enrichment and identification of human 'fetal' epidermal stem cells.
Zhou JX, Chen SY, Liu WM, Cao YJ, Duan EK
Human reproduction (Oxford, England) 2004 Apr;19(4):968-74
Human reproduction (Oxford, England) 2004 Apr;19(4):968-74
Alterations of beta-catenin pathway in non-melanoma skin tumors: loss of alpha-ABC nuclear reactivity correlates with the presence of beta-catenin gene mutation.
Doglioni C, Piccinin S, Demontis S, Cangi MG, Pecciarini L, Chiarelli C, Armellin M, Vukosavljevic T, Boiocchi M, Maestro R
The American journal of pathology 2003 Dec;163(6):2277-87
The American journal of pathology 2003 Dec;163(6):2277-87
Wnt signaling controls the phosphorylation status of beta-catenin.
van Noort M, Meeldijk J, van der Zee R, Destree O, Clevers H
The Journal of biological chemistry 2002 May 17;277(20):17901-5
The Journal of biological chemistry 2002 May 17;277(20):17901-5
Wnt signals are transmitted through N-terminally dephosphorylated beta-catenin.
Staal FJ, Noort Mv Mv, Strous GJ, Clevers HC
EMBO reports 2002 Jan;3(1):63-8
EMBO reports 2002 Jan;3(1):63-8
No comments: Submit comment
Supportive validation
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- WB: 20 micrograms of A431 cell lysate was probed with anti-Active-_-Catenin, clone 8E7. Arrow indicates _-catenin (92kDa).
- Validation comment
- WB
Supportive validation
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- Representative images from a previous lot. Confocal fluorescent analysis of Active-_-Catenin using 05-665 (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Antibody shows positive staining on the membrane and cytosol.
- Validation comment
- ICC
