NBP1-32239

antibody from Novus Biologicals

Targeting: CTNNB1 armadillo, beta-catenin, CTNNB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Proximity ligation assay (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-32239

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-32239, RRID:AB_10003922

Product name

Rabbit Polyclonal beta-Catenin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Cadherins in the retinal pigment epithelium (RPE) revisited: P-cadherin is the highly dominant cadherin expressed in human and mouse RPE in vivo.

Yang X, Chung JY, Rai U, Esumi N
PloS one 2018;13(1):e0191279

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WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation.

Xu M, Horrell J, Snitow M, Cui J, Gochnauer H, Syrett CM, Kallish S, Seykora JT, Liu F, Gaillard D, Katz JP, Kaestner KH, Levin B, Mansfield C, Douglas JE, Cowart BJ, Tordoff M, Liu F, Zhu X, Barlow LA, Rubin AI, McGrath JA, Morrisey EE, Chu EY, Millar SE
Nature communications 2017 Jun 7;8:15397

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Environmental Enrichment Improves Spatial Learning and Memory in Vascular Dementia Rats with Activation of Wnt/β-Catenin Signal Pathway.

Jin X, Li T, Zhang L, Ma J, Yu L, Li C, Niu L
Medical science monitor : international medical journal of experimental and clinical research 2017 Jan 13;23:207-215

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Environmental Enrichment Improves Spatial Learning and Memory in Vascular Dementia Rats with Activation of Wnt/β-Catenin Signal Pathway.

Jin X, Li T, Zhang L, Ma J, Yu L, Li C, Niu L
Medical science monitor : international medical journal of experimental and clinical research 2017 Jan 13;23:207-215

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: beta-Catenin Antibody [NBP1-32239] - Western blot for beta-catenin in HEK 293 cells treated with LiCl (10mM) for 1 hr. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: beta-Catenin Antibody [NBP1-32239] - A. 30 ug PC-12 whole cell lysate/extract 7.5 % SDS-PAGE beta Catenin antibody [N1N2-2], N-term dilution: 1:1000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: beta-Catenin Antibody [NBP1-32239] - Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: beta-Catenin Antibody [NBP1-32239] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: beta-Catenin Antibody [NBP1-32239] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term diluted at 1:3000.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse colon. Green: beta Catenin antibody [diluted at 1:500. Red: alpha Tubulin antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse urinary bladder diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse colon dilution: 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse skin dilution: 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded human esophagus. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse intestine. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded rat duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded human cervix. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded rat colon. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - WNT10A/beta-catenin signaling is required for region-specific differentiation. Epithelial deletion of beta-catenin (g-h",k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta-catenin, LEF1 and HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms15397), licensed under a CC-BY licence.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: beta-Catenin Antibody [NBP1-32239] - HeLa cell.Black: Unlabelled sample was used as a control. Red: beta Catenin antibody [N1N2-2], N-term dilution: 1:50.Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP): beta-Catenin Antibody [NBP1-32239] - Cross-linked ChIP was performed with HCT116 chromatin extract and 5 ug of either control rabbit IgG or anti-beta Catenin antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.