NBP2-03615

antibody from Novus Biologicals

Targeting: CTNNB1 armadillo, beta-catenin, CTNNB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-03615

Provider

Novus Biologicals

Product name

Mouse Monoclonal beta-Catenin Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Rat, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: beta-Catenin Antibody (12H7) [NBP2-03615] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta Catenin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-beta Catenin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: beta-Catenin Antibody (12H7) [NBP2-03615] - Analysis of extracts (35ug) from 9 different cell lines by using anti-beta Catenin monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-beta Catenin mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: beta-Catenin Antibody (12H7) [NBP2-03615] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-beta Catenin antibody, and then analyzed by flow cytometry.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: beta-Catenin Antibody (12H7) [NBP2-03615] - Analysis of Hela cells, using anti-beta Catenin antibody, (Red), compared to a nonspecific negative control antibody (Blue).