MA1-10056

antibody from Invitrogen Antibodies

Targeting: CTNNB1 armadillo, beta-catenin, CTNNB

Immunoprecipitation

Antibody data

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Validation data

Product number: MA1-10056 - Provider product page
Provider: Invitrogen Antibodies
Product name: beta Catenin Monoclonal Antibody (EM-22)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: This antibody reacts with C-terminal part of beta-catenin (intracellular antigen), an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.
Reactivity: Human, Mouse, Hamster
Host: Mouse
Isotype: IgG
Antibody clone number: EM-22
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: 4° C, do not freeze

CTNNB1 protein structure - MA1-10056 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blotting analysis of beta-catenin in murine 3T3 (A), C57 (B) and KW1 (C) cell lines using EM-22 antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blotting analysis of beta-catenin in murine 3T3 (A), C57 (B) and KW1 (C) cell lines using EM-22 Monoclonal antibody (Product # MA1-10056).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-beta Catenin Monoclonal Antibody (EM-22) (Product # MA1-10056) and a 85 kDa band corresponding to Catenin beta-1 was observed across the cell lines and tissues tested except Hep G2 which contain an exon 3-4 truncation mutation in the CTNNB1 gene corresponding to deletion of amino acids 25-140 in the ß-catenin protein. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), SK-MEL-31 (Lane 2), MCF7 (Lane 3), MCF7 treated with 10 ng/mL of TGF-ß1 for 24 hours (Lane 4), A-431 (Lane 5), HeLa (Lane 6) and Mouse Lung (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry staining of beta-catenin in human colon adenocarcinoma cell line HT29 using EM-22antibody (green). Cell nuclei visualized by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry staining of beta-catenin in human colon adenocarcinoma cell line HT29 using EM-22 Monoclonal antibody(green) (Product # MA1-10056). Cell nuclei visualized by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Catenin beta-1 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with beta Catenin Monoclonal Antibody (EM-22) (Product # MA1-10056) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing membrane localization. Panel e represents decreased expression of Beta catenin -1 in MCF7 cells treated with 10 ng/mL of TGF-ß1 for 24 hours. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.