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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-15569 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta Catenin Monoclonal Antibody (4D5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15569 targets CTNNB1 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 4D5
- Concentration
- Conc. Not Determined
Submitted references Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT.
Aban CE, Lombardi A, Neiman G, Biani MC, La Greca A, Waisman A, Moro LN, Sevlever G, Miriuka S, Luzzani C
Scientific reports 2021 Jan 21;11(1):2048
Scientific reports 2021 Jan 21;11(1):2048
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CTNNB1 using CTNNB1 monoclonal antibody (Product # MA5-15569) in CTNNB1-human IgG Fc transfected HEK293 cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of A549 (left) and SK-BR-3 (right) cells using CTNNB1 monoclonal antibody (Product # MA5-15569) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human placenta tissues using CTNNB1 monoclonal antibody (Product # MA5-15569).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A549 cells using CTNNB1 monoclonal antibody (Product # MA5-15569) (green) and negative control (purple).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 E-cadherin decrease activates the initiation of EMT. qPCR analysis of ( a ) genes involved in EMT such as SNAI1 , SNAI2 , ZEB1 and ZEB2 and ( b ) genes of early mesoderm precursor TBX6 and MIXL1 when E-cadherin is downregulated. ( c ) Relative expression level of LINC-ROR and MALAT1 , two lnc-RNAs that act as regulators of EMT were measured by qPCR. AU is the abbreviation for arbitrary units. Results are represented as mean +- SD (n = 5). ANOVA with post hoc Tukey. Different letters indicate significant differences of groups compared to control condition (p < 0.05). ( d ) Immunofluorescence and quantification of mean fluorescence intensity of beta-catenin (grey) in cells treated with Dox. Nuclei were stained with DAPI (blue). Magnification of dotted areas are shown in black and white colors. Scale bar 50 um. Representative images of at least three experiments are shown. Results are represented as mean +- SD (n = 3). ANOVA with post hoc Tukey. Different letters indicate significant differences between groups (p < 0.05). ( e ) Relative mRNA levels of c-myc ( MYCBP ) and cyclin D1 ( CCND1 ) were determined by qPCR. Data are presented as mean +- SD (n = 4). ANOVA with post hoc Tukey. Different letters indicate significant differences of groups compared to control condition (p < 0.05).
