Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-16429 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta Catenin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-16429 targets Catenin-beta in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with mouse, rat, Xenopus, chicken, and human samples.
- Concentration
- 1 mg/mL
Submitted references Cardamonin inhibits colonic neoplasia through modulation of MicroRNA expression.
James S, Aparna JS, Paul AM, Lankadasari MB, Mohammed S, Binu VS, Santhoshkumar TR, Reshmi G, Harikumar KB
Scientific reports 2017 Oct 24;7(1):13945
Scientific reports 2017 Oct 24;7(1):13945
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CTNNB1 was performed with 10 µg of A549 cells transfected with Transfection Reagent alone (Lane 1), 100nM Non-Targeting control siRNA (Lane 2), or 100nM siRNA against CTNNB1 (Lane 3). Proteins were resolved using a NuPAGE® Novex 4-12% Bis-Tris Gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and a protein size ladder. Proteins were wet transferred to a Pierce Nitrocellulose Membrane (Product # 88025) OR Pierce PVDF Membrane (Product # 88518) and blocked with Pierce Starting Block T20 (PBS) Blocking Buffer (Product # 37539) for 1 hour at room temperature. CTNNB1 was detected at ~ 92 kDa using CTNNB1 Rabbit polyclonal antibody (Product # PA5-16429) diluted in Pierce Starting Block T20 (PBS) Blocking Buffer 4°C overnight on a rocking platform. Pierce Goat Anti-Rabbit (Product # 31461) HRP-Conjugated Antibodies at a 1:2500 dilution were used and chemiluminescent detection was performed using Pierce Supersignal West Dura Maximum Sensitivity Substrate (Product # 37071). Relative density of the bands normalized to GAPDH (36 kDa). CTNNB1 Antibody (Product # PA5-16429) confirms silencing of CTNNB1 expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Catenin-beta using Catenin-beta Polyclonal Antibody (Product # PA5-16429) on MCF-7 Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Catenin-beta using Catenin-beta Polyclonal Antibody (Product # PA5-16429) on MCF-7 Cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human breast cancer stained with Catenin, beta antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and membrane staining of tumor cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Catenin-beta using Catenin-beta Polyclonal Antibody (Product # PA5-16429) on Native Human LNCaP Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Catenin-beta using Catenin-beta Polyclonal Antibody (Product # PA5-16429) on Native Human LNCaP Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Cardamonin inhibited AOM induced colorectal cancer: ( a ) Chemical structure of cardamonin; (b) Schematic representation of experimental design; (c) Number of tumors in colon/ group; (d) Mean tumor size between groups expressed as mean +- SEM. The statistical analysis was done using one way ANOVA followed by Tukey HSD posthoc test; ***p < 0.001; (e) Tissue sections were prepared from formalin fixed paraffin embedded samples. Hematoxylin-eosin staining (upper panel) and Ki-67 index (lower panel) of colonic tissue samples, representative images, 40X magnification; (f) Nuclear fractions were prepared from all the 4 groups (n = 3) and western blot analysis was performed for beta-catenin (upper panel) and p65 (lower panel). Lamin A/C was used as the loading control.
