Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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Validation data
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- Product number
- ALX-804-260-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-260-C100, RRID:AB_11181465
- Product name
- _-Catenin monoclonal antibody (8E4)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Thiophilic adsorption and size exclusion chromatography purified.
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8E4
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Submitted references An aberrant nuclear localization of E-cadherin is a potent inhibitor of Wnt/β-catenin-elicited promotion of the cancer stem cell phenotype.
Wnt signaling and phosphorylation status of beta-catenin: importance of the correct antibody tools.
Sulindac targets nuclear beta-catenin accumulation and Wnt signalling in adenomas of patients with familial adenomatous polyposis and in human colorectal cancer cell lines.
Illegitimate WNT signaling promotes proliferation of multiple myeloma cells.
Activation of the canonical beta-catenin pathway by histamine.
Modulation of intracellular beta-catenin localization and intestinal tumorigenesis in vivo and in vitro by sphingolipids.
Su YJ, Chang YW, Lin WH, Liang CL, Lee JL
Oncogenesis 2015 Jun 15;4:e157
Oncogenesis 2015 Jun 15;4:e157
Wnt signaling and phosphorylation status of beta-catenin: importance of the correct antibody tools.
van Noort M, Weerkamp F, Clevers HC, Staal FJ
Blood 2007 Oct 1;110(7):2778-9
Blood 2007 Oct 1;110(7):2778-9
Sulindac targets nuclear beta-catenin accumulation and Wnt signalling in adenomas of patients with familial adenomatous polyposis and in human colorectal cancer cell lines.
Boon EM, Keller JJ, Wormhoudt TA, Giardiello FM, Offerhaus GJ, van der Neut R, Pals ST
British journal of cancer 2004 Jan 12;90(1):224-9
British journal of cancer 2004 Jan 12;90(1):224-9
Illegitimate WNT signaling promotes proliferation of multiple myeloma cells.
Derksen PW, Tjin E, Meijer HP, Klok MD, MacGillavry HD, van Oers MH, Lokhorst HM, Bloem AC, Clevers H, Nusse R, van der Neut R, Spaargaren M, Pals ST
Proceedings of the National Academy of Sciences of the United States of America 2004 Apr 20;101(16):6122-7
Proceedings of the National Academy of Sciences of the United States of America 2004 Apr 20;101(16):6122-7
Activation of the canonical beta-catenin pathway by histamine.
Diks SH, Hardwick JC, Diab RM, van Santen MM, Versteeg HH, van Deventer SJ, Richel DJ, Peppelenbosch MP
The Journal of biological chemistry 2003 Dec 26;278(52):52491-6
The Journal of biological chemistry 2003 Dec 26;278(52):52491-6
Modulation of intracellular beta-catenin localization and intestinal tumorigenesis in vivo and in vitro by sphingolipids.
Schmelz EM, Roberts PC, Kustin EM, Lemonnier LA, Sullards MC, Dillehay DL, Merrill AH Jr
Cancer research 2001 Sep 15;61(18):6723-9
Cancer research 2001 Sep 15;61(18):6723-9
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Figure A: Western blot analysis of recombinant _-catenin from E. coli (mainly depospho-_-catenin) using various antibodies.. Figure B: Western blot analysis of lysate of pervanadate-treated SKOV-3 cells (phospho- and dephospho-_-catenin) using various antibodies. Method: _-catenin probes were applied to SDS-PAGE, transferred to PVDF membranes, probed for 1 hour at 15-22¡C with the indicated MAbs (0.5µg/ml)Êand developed with ECL (exposure time 5 sec.). Antibodies used are: . Lane 1: MAb to _-Catenin (dephospho, aa 35-50) (7A7)Ê (Prod. No. ALX-804-259). Lane 2: MAb to _-Catenin (NT/Exon 2) (7D11) (Prod. No. ALX-804-060). Lane 3: MAb to _-Catenin (dephospho, aa 27-37) (8E4) (Prod. No. ALX-804-260). Lane 4: MAb to _-Catenin (Exon 3) (9G2) (Prod. No. ALX-804-058)
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Detection of endogenous _-Catenin.Whole cell lysates of serum starved tumor cells (20'000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with MAb to _-Catenin (8E4) (Prod. No. ALX-804-260/1) (0.5 µg/ml) for 1h at RT and developed by ECL (exp. time: 30s). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D