ALX-804-060-C100

antibody from Enzo Life Sciences

Targeting: CTNNB1 armadillo, beta-catenin, CTNNB

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ALX-804-060-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-060-C100, RRID:AB_2050840

Product name

_-Catenin (NT/exon 2) monoclonal antibody (7D11)

Antibody type

Monoclonal

Antigen

Recombinant full length protein

Description

Thiophilic adsorption and size exclusion chromatography purified.

Reactivity

Human, Mouse, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

7D11

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.

References

Submitted references

STAT3 paradoxically stimulates β-catenin expression but inhibits β-catenin function.

Ibrahem S, Al-Ghamdi S, Baloch K, Muhammad B, Fadhil W, Jackson D, Nateri AS, Ilyas M
International journal of experimental pathology 2014 Dec;95(6):392-400

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Relation between expression pattern of wild-type p53 and multidrug resistance proteins in human nephroblastomas.

Hodorová I, Rybárová S, Vecanová J, Solár P, Plank L, Mihalik J
Acta histochemica 2013 Apr;115(3):273-8

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Phospho-beta-catenin accumulation in Alzheimer's disease and in aggresomes attributable to proteasome dysfunction.

Ghanevati M, Miller CA
Journal of molecular neuroscience : MN 2005;25(1):79-94

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The human F box protein beta-Trcp associates with the Cul1/Skp1 complex and regulates the stability of beta-catenin.

Latres E, Chiaur DS, Pagano M
Oncogene 1999 Jan 28;18(4):849-54

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Beta-catenin mutations in cell lines established from human colorectal cancers.

Ilyas M, Tomlinson IP, Rowan A, Pignatelli M, Bodmer WF
Proceedings of the National Academy of Sciences of the United States of America 1997 Sep 16;94(19):10330-4

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beta-catenin is a target for the ubiquitin-proteasome pathway.

Aberle H, Bauer A, Stappert J, Kispert A, Kemler R
The EMBO journal 1997 Jul 1;16(13):3797-804

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Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric carcinoma: relationship with patient survival.

Jawhari A, Jordan S, Poole S, Browne P, Pignatelli M, Farthing MJ
Gastroenterology 1997 Jan;112(1):46-54

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XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos.

Molenaar M, van de Wetering M, Oosterwegel M, Peterson-Maduro J, Godsave S, Korinek V, Roose J, Destrée O, Clevers H
Cell 1996 Aug 9;86(3):391-9

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Functional interaction of beta-catenin with the transcription factor LEF-1.

Behrens J, von Kries JP, Kühl M, Bruhn L, Wedlich D, Grosschedl R, Birchmeier W
Nature 1996 Aug 15;382(6592):638-42

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Regulation of intracellular beta-catenin levels by the adenomatous polyposis coli (APC) tumor-suppressor protein.

Munemitsu S, Albert I, Souza B, Rubinfeld B, Polakis P
Proceedings of the National Academy of Sciences of the United States of America 1995 Mar 28;92(7):3046-50

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Beta-catenin mediates the interaction of the cadherin-catenin complex with epidermal growth factor receptor.

Hoschuetzky H, Aberle H, Kemler R
The Journal of cell biology 1994 Dec;127(5):1375-80

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Responses to Wnt signals in vertebrate embryos may involve changes in cell adhesion and cell movement.

Moon RT, DeMarais A, Olson DJ
Journal of cell science. Supplement 1993;17:183-8

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The product of the Drosophila segment polarity gene armadillo is part of a multi-protein complex resembling the vertebrate adherens junction.

Peifer M
Journal of cell science 1993 Aug;105 ( Pt 4):993-1000

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Figure A: Western blot analysis of recombinant _-catenin from E. coli (mainly depospho-_-catenin) using various antibodies.. Figure B: Western blot analysis of lysate of pervanadate-treated SKOV-3 cells (phospho- and dephospho-_-catenin) using various antibodies. Method: _-catenin probes were applied to SDS-PAGE, transferred to PVDF membranes, probed for 1 hour at 15-22¡C with the indicated MAbs (0.5µg/ml)Êand developed with ECL (exposure time 5 sec.). Antibodies used are: . Lane 1: MAb to _-Catenin (dephospho, aa 35-50) (7A7)Ê (Prod. No. ALX-804-259). Lane 2: MAb to _-Catenin (NT/Exon 2) (7D11) (Prod. No. ALX-804-060). Lane 3: MAb to _-Catenin (dephospho, aa 27-37) (8E4) (Prod. No. ALX-804-260). Lane 4: MAb to _-Catenin (Exon 3) (9G2) (Prod. No. ALX-804-058)