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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX80546 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX80546, RRID:AB_11175655
- Product name
- SH2D1B antibody, Internal
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Purified through a protein A column, followed by peptide affinity purification.
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of SH2D1B Antibody (Center) (GTX80546) in Jurkat cell line lysates (35ug/lane). SH2D1B (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SH2D1B Antibody (Center) (GTX80546), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SH2D1B Antibody (Center) (GTX80546) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
